学龄儿童肠道直肠真杆菌和乳酸杆菌分布特征分析

Intestinal distribution of Eubacterium rectale and Lactobacillus among school-age children

目的探讨正常体重、超重和肥胖学龄儿童肠道直肠真杆菌和乳酸杆菌的分布特征,分析超重和肥胖儿童肠道微生态失衡特点,为通过调节肠道微生态防治肥胖提供理论依据。方法 2017年11—12月以江苏省南京市某小学全体学生为调查对象,应用实时荧光定量PCR法测定166份儿童粪便样本,分析正常体重、超重和肥胖学龄儿童肠道中直肠真杆菌和乳酸杆菌的分布。结果直肠真杆菌数量在正常体重组、超重组和肥胖组呈逐渐升高的趋势,差异均具有统计学意义(P<0.05)。乳酸杆菌数量在正常体重组、超重组和肥胖组呈逐渐降低的趋势,且差异均具有统计学意义(P<0.05)。正常体重、超重和肥胖儿童肠道中直肠真杆菌和乳酸杆菌的分布与性别无关,除正常体重组乳酸杆菌随年龄的增加而降低外(P<0.05),其余组差异均不具有统计学意义(P>0.05)。结论直肠真杆菌数量的增加与乳酸杆菌数量的减少可能与超重和肥胖的发生发展有关,正常体重、超重和肥胖学龄儿童肠道中直肠真杆菌和乳酸杆菌的分布受性别和年龄影响较小。

Objective To analyze intestinal distribution of Eubacterium recta le and Lactobacillus in normal-weight,overweight and obese school-age children and the characteristics of intestinal flora imbalance in overweight and obese children and to provide a theoretical basis for the prevention and control of obesity through intestinal microecological regulation. Methods We collected 166 feces samples from all pupils in a primary school in Nanjing city of Jiangsu province for detections of Eubacterium rectale and Lactobacillus with real-time quantitative PCR method. Results Significant differences in the number of Eubacterium rectale and Lactobacillus were observed among the three groups of children, with a higher number in the obese children and a lower number in the normal-weight children for Eubacterium rectale(P < 0.05)but a higher number in the normal-weight children and a lower number in the obese children for Lactobacillus(P <0.05),respectively. No significant gender difference in the number of intestinal Eubacterium rectale and Lactobacillus was observed among all the three groups of children. The number of Lactobacillus decreased significantly with the increment of age only in the normal-weight children(P < 0.05) but no age difference was observed in the number of intestinal Eubacterium rectale among the three groups and in Lactobacillus number in overweight and obese children. Conclusion The increase in the number of Eubacterium rectale and the decrease in the number of intestinal Lactobacillus may be related to the occurrence and development of overweight and obesity in school age children. The distribution of Eubacterium rectale and Lactobacillus in the intestine of normal weight, overweight and obese children are less affected by children’s gender and age.