PDMS-Ag基底表面增强拉曼光谱技术快速检测鱼肉中孔雀石绿

Rapid detection of malachite green in fish by PDMS-Ag surface enhanced Raman spectroscopy

采用葡萄糖还原硝酸银,并在聚二甲基硅氧烷(PDMS)薄片表面沉积银纳米颗粒的方法,制备表面增强拉曼(SERS)基片。研究该SERS基片对孔雀石绿(MG)的拉曼光谱分析的增强效能。结果表明,该方法生成的PDMS-Ag复合基底对MG的检测线性范围为1×10^(-10)～1×10^(-5)mol/L,线性相关系数R^2为0. 9796。其对孔雀石绿的最低检测浓度1×10^(-10)mol/L,SERS谱图MG特征峰型清晰易分辨。将本方法应用到鲫鱼鱼肉样品中MG含量的检测,孔雀石绿加标回收率为96. 4%～128. 4%,相对标准偏差(RSD)为5. 9%～8. 2%。

A surface-enhanced Raman spectroscopy (SERS)substrate was prepared by reducing silver nitrate with glucose and depositing silver nanoparticles on the surface of a polydimethylsiloxane (PDMS)wafer.The enhanced performance of this SERS substrate for Raman spectroscopic analysis of malachite green (MG)was investigated.The results showed that the linear range of detection of MG by PDMS-Ag composite substrate generated by this method was 1×10^-10 to 1×10^-5 mol/L and the linear correlation coefficient R^2 was 0.9796.The minimum detectable concentration of malachite green was 1 ×10^-10 mol/L,and the MG feature peak shape of the SERS spectrum can be clearly and easily distinguished.The method was applied to the detection of MG content in carp fish samples.The recoyeries of malachite green were 96.4%-128.4% in the spiked samples,and the relative standard deviations (RSD)were 5.9%-8.2%.