摘要
目的探讨微小RNA-574-5p(miR-574-5p)在胃癌组织中的表达水平及临床意义。方法选取2010年10月至2017年10月武汉大学中南医院收治的胃癌患者胃癌组织和癌旁组织标本64例。采用实时荧光定量技术(RT-PCR)检测标本中miR-574-5p的表达量。结果胃癌组织中miR-574-5p表达量为(2.37±0.69)ng/μL,癌旁组织中miR-574-5p表达量为(0.36±0.11)ng/μL,差异有统计学意义(t=25.32,P=0.000)。肿瘤大小>5cm[(3.04±0.74)ng/μL]、TNM分期Ⅲ~Ⅳ期[(3.36±0.76)ng/μL]、浸润深度T3~T4[(3.29±0.72)ng/μL]、低分化[(3.34±0.74)ng/μL]、出现远处转移[(3.39±0.78)ng/μL]的胃癌患者,miR-574-5p的表达水平高于肿瘤大小≤5cm[(2.05±0.68)ng/μL]、TNM分期Ⅰ~Ⅱ期[(2.02±0.61)ng/μL]、浸润深度T1~T2[(2.07±0.64)ng/μL]、中高分化[(2.04±0.65)ng/μL]、未出现远处转移[(2.01±0.68)ng/μL]的胃癌患者,差异均有统计学意义(t=4.29、4.68、4.52、4.62、4.79,均P<0.01)。Kaplan-meier分析显示,胃癌组织中miR-574-5p高表达的患者,5年生存率仅为16%,远低于miR-574-5p低表达的患者的5年生存率(58%),差异有统计学意义(χ^2=5.872,P=0.001)。结论胃癌组织中miR-574-5p呈高表达,胃癌组织中miR-574-5p的表达水平与肿瘤大小、TNM分期、浸润深度、分化程度、远处转移有关,miR-574-5p高表达的患者其5年生存率较低。
Objective To investigate the expression level and clinical significance of micro RNA-574-5p (miR-574-5p) in gastric carcinoma. Methods From October 2010 to October 2017, 64 specimens of gastric cancer and adjacent tissues from Zhongnan Hospital were collected.Real time fluorescence quantitative technique (RT-PCR) was used to detect the expression of miR-574-5p in specimens. Results The expression of miR-574-5p in gastric cancer tissues was (2.37±0.69)ng/μL, and the expression of miR-574-5p in the para cancerous tissues was (0.36±0.11)ng/μL, the difference was statistically significant (t=25.32, P=0.000). The expression levels of miR-574-5p in patients with the size of tumor size>5cm[(3.04±0.74)ng/μL], TNM staging Ⅲ-Ⅳ[(3.36±0.76)ng/μL], depth of invasion T3-T4[(3.29±0.72)ng/μL], low differentiation[(3.34±0.74)ng/μL], and distant metastasis[(3.39±0.78)ng/μL], were higher than those of tumor size less than 5cm[(2.05±0.68)ng/μL], TNM stage Ⅰ-Ⅱ phase[(2.02±0.61)ng/μL], depth of invasion T1-T2[(2.07±0.64)ng/μL], middle and high differentiation[(2.04±0.65)ng/μL]and no distant metastasis[(2.01±0.68)ng/μL], the differences were statistically significant (t=4.29, 4.68, 4.52, 4.62, 4.79, all P<0.01). Kaplan-meier analysis showed that the 5-year survival rate of patients with high expression of miR-574-5p in gastric cancer was only 16%, which was significantly lower than that in patients with low expression of miR-574-5p (58%), and the difference was statistically significant (χ^2=5.872, P=0.001). Conclusion miR-574-5p expression in gastric carcinoma is high.The level of miR-574-5p expression in gastric carcinoma is related to the size of tumor, TNM stage, depth of invasion, degree of differentiation and distant metastasis.The 5-year survival rate of the patients with high expression of miR-574-5p is low.
作者
张芬
Zhang Fen(Department of Pathology ,Zhongnan Hospital,Wuhan University,Wuhan ,Hubei 430071,China)
出处
《中国基层医药》
CAS
2019年第1期44-47,共4页
Chinese Journal of Primary Medicine and Pharmacy
关键词
胃肿瘤
核酸类
病理学
临床
肿瘤分期
肿瘤转移
细胞分化
淋巴细胞
肿瘤浸润
Stomach neoplasms
Nucleic acids
Pathology, clinical
Neoplasm staging
Neoplasm metastasis
Cell differentiation
Lymphocyte, tumor-infiltrating