微小RNA-574-5p在胃癌组织中的表达及意义

Clinical significance and expression of miR-574 -5p in patients with gastric cancer

目的探讨微小RNA-574-5p(miR-574-5p)在胃癌组织中的表达水平及临床意义。方法选取2010年10月至2017年10月武汉大学中南医院收治的胃癌患者胃癌组织和癌旁组织标本64例。采用实时荧光定量技术(RT-PCR)检测标本中miR-574-5p的表达量。结果胃癌组织中miR-574-5p表达量为(2.37±0.69)ng/μL,癌旁组织中miR-574-5p表达量为(0.36±0.11)ng/μL,差异有统计学意义(t=25.32,P=0.000)。肿瘤大小>5cm[(3.04±0.74)ng/μL]、TNM分期Ⅲ~Ⅳ期[(3.36±0.76)ng/μL]、浸润深度T3~T4[(3.29±0.72)ng/μL]、低分化[(3.34±0.74)ng/μL]、出现远处转移[(3.39±0.78)ng/μL]的胃癌患者,miR-574-5p的表达水平高于肿瘤大小≤5cm[(2.05±0.68)ng/μL]、TNM分期Ⅰ~Ⅱ期[(2.02±0.61)ng/μL]、浸润深度T1~T2[(2.07±0.64)ng/μL]、中高分化[(2.04±0.65)ng/μL]、未出现远处转移[(2.01±0.68)ng/μL]的胃癌患者,差异均有统计学意义(t=4.29、4.68、4.52、4.62、4.79,均P<0.01)。Kaplan-meier分析显示,胃癌组织中miR-574-5p高表达的患者,5年生存率仅为16%,远低于miR-574-5p低表达的患者的5年生存率(58%),差异有统计学意义(χ^2=5.872,P=0.001)。结论胃癌组织中miR-574-5p呈高表达,胃癌组织中miR-574-5p的表达水平与肿瘤大小、TNM分期、浸润深度、分化程度、远处转移有关,miR-574-5p高表达的患者其5年生存率较低。

Objective To investigate the expression level and clinical significance of micro RNA-574-5p (miR-574-5p) in gastric carcinoma. Methods From October 2010 to October 2017, 64 specimens of gastric cancer and adjacent tissues from Zhongnan Hospital were collected.Real time fluorescence quantitative technique (RT-PCR) was used to detect the expression of miR-574-5p in specimens. Results The expression of miR-574-5p in gastric cancer tissues was (2.37±0.69)ng/μL, and the expression of miR-574-5p in the para cancerous tissues was (0.36±0.11)ng/μL, the difference was statistically significant (t=25.32, P=0.000). The expression levels of miR-574-5p in patients with the size of tumor size>5cm[(3.04±0.74)ng/μL], TNM staging Ⅲ-Ⅳ[(3.36±0.76)ng/μL], depth of invasion T3-T4[(3.29±0.72)ng/μL], low differentiation[(3.34±0.74)ng/μL], and distant metastasis[(3.39±0.78)ng/μL], were higher than those of tumor size less than 5cm[(2.05±0.68)ng/μL], TNM stage Ⅰ-Ⅱ phase[(2.02±0.61)ng/μL], depth of invasion T1-T2[(2.07±0.64)ng/μL], middle and high differentiation[(2.04±0.65)ng/μL]and no distant metastasis[(2.01±0.68)ng/μL], the differences were statistically significant (t=4.29, 4.68, 4.52, 4.62, 4.79, all P<0.01). Kaplan-meier analysis showed that the 5-year survival rate of patients with high expression of miR-574-5p in gastric cancer was only 16%, which was significantly lower than that in patients with low expression of miR-574-5p (58%), and the difference was statistically significant (χ^2=5.872, P=0.001). Conclusion miR-574-5p expression in gastric carcinoma is high.The level of miR-574-5p expression in gastric carcinoma is related to the size of tumor, TNM stage, depth of invasion, degree of differentiation and distant metastasis.The 5-year survival rate of the patients with high expression of miR-574-5p is low.