载氨基化碳纳米管壳聚糖/β-甘油磷酸钠温敏凝胶对NGF的缓释性及释药机制研究

Study on sustained release and release mechanism of nerve growth factor from chitosan/β-glycerophosphate thermosensitive hydrogel containing amino-carbon nanotubes

目的:考察载氨基化碳纳米管的壳聚糖/β-甘油磷酸钠(CS/β-GP)温敏凝胶对神经生长因子(NGF)的释药机制。方法:以NGF为模型药物,考察载氨基化碳纳米管的CS/β-GP温敏凝胶的缓释性,并采用零级方程、一级方程和Higuchi方程、Hixson-Crowell、Weibull和Peppas方程等各释药模型初步探讨其释药机制。结果:载氨基化碳纳米管的CS/β-GP温敏凝胶对NGF有较好的缓释作用,释放行为与凝胶微观结构有一定关系。NGF在壳聚糖温敏凝胶系统中的体外释放行为与Weibull模型最接近,即以扩散和溶蚀为主的释药机制。结论:氨基化碳纳米管的CS/β-GP温敏凝胶释药过程是药物扩散和凝胶降解的双重结果,从而为CS/β-GP温敏凝胶的缓释性研究提供了一定基础。

Objective: To investigate the release mechanism of nerve growth factor( NGF) from chitosan/β-glycerine phosphate( CS/β-GP) thermosensitive hydrogel containing amino-carbon nanotubes. Methods: The sustained release of CS/β-GP thermosensitive hydrogel containing amino-carbon nanotubes was studied by using nerve growth factor( NGF) as model drug,and the mechanism of drug release was preliminarily explored by the zero-level equation,first-order equation and Higuchi equation,Hixson-crowell,Weibull and Peppas equations.Results: The CS/β-GP thermosensitive hydrogel containing amino-carbon nanotubes had a good sustained release effect on NGF,and the release behavior was related to the gel microstructure. The in vitro release behavior of NGF in the chitosan thermosensitive hydrogel system was closest to the Weibull model,that is,diffusion and dissolution are the main mechanisms of drug release. Conclusion: The release process of CS/β-GP thermosensitive hydrogel containing amino-carbon nanotubes is a combined result of drug diffusion and gel degradation,which provides a foundation for the study of CS/β-GP thermosensitive hydrogel.