高效液相色谱法测定水果罐头中阿斯巴甜的不确定度评估

Uncertainty evaluation for determination of aspartame in canned fruits by high performance liquid chromatography

目的评定高效液相色谱法测定水果罐头中阿斯巴甜含量的不确定度。方法按照GB 5009.263-2016《食品安全国家标准食品中阿斯巴甜和阿力甜的测定》测定水果罐头中阿斯巴甜,根据JJF 1059.1-2012《测量不确定度评定与表示》的方法,建立高效液相色谱法测定水果罐头中阿斯巴甜不确定度评定的数学模型,分析其中各不确定度分量的来源,并对其分别进行量化,最终合成水果罐头中阿斯巴甜含量测定结果的不确定度。结果水果罐头中阿斯巴甜含量测定结果 0.0926 g/kg时,在95%的置信区间下,扩展不确定度为0.00282g/kg(k=2)。结论实验过程的不确定度主要来源引有标准溶液的配制操作、标准曲线的拟合过程、试样定容、重复性测定等。

Objective To evaluate the uncertainty of the determination of aspartame content in canned fruits by high performance liquid chromatography(HPLC). Methods According to GB 5009.263-2016 National food safety standard-Determination of aspartame and alitream in food, the content of aspartame in canned fruits was detected. According to JJF 1059.1-2012 Evaluation and expression of measurement uncertainty, a mathematical model for the uncertainty of determination of aspartame in canned fruits by HPLC was established. The source of each component of uncertainty was analyzed and quantified, and the uncertainty of determination of aspartame content in canned fruits was finally synthesized. Results When the aspartame content was 0.0926 g/kg, the extended uncertainty was 0.00282 g/kg(k=2) under the 95% confidence interval. Conclusion The evaluation results show that the uncertainties of the experiment are mainly derived from the preparation of standard solution, standard curve fitting process, sample constant volume and the repeatability measurement.