高效液相色谱法测定多环芳烃的出峰顺序探讨

Study on the peak sequence of determination of polycyclic aromatic hydrocarbons by high performance liquid chromatography

目的探讨水﹑气﹑土壤中多环芳烃检测标准(HJ 478-2009﹑HJ 647-2013、HJ 784-2016)的正确出峰时间和顺序。方法用高效液相色谱来对苊烯、芴、苊、?、苯并(a)蒽进行定性分析,并与3个标准中的出峰顺序进行比较。结果苊烯、苊、芴、苯并(a)蒽、?的出峰时间分别为6.450、7.923、8.233、17.760、18.740min,与标准HJ478-2009﹑HJ647-2013的出峰顺序存在差异。结论在使用标准HJ478-2009﹑HJ647-2013、HJ 784-2016同时测定16种多环芳烃时,多环芳烃的出峰顺序及时间应以HJ 784-2016为准。

Objective To study the correct peak time and sequence of detection standards(HJ 478-2009, HJ 647-2013, HJ 784-2016) for polycyclic aromatic hydrocarbons in water, gas and soil. Methods High performance liquid chromatography was used to qualitatively analyze terpenes, ruthenium, osmium, iridium, and benzo(a)pyrene, and compared with the peak order in the 3 standards. Results The peak times of terpenes, anthracene, anthracene, benzo(a)pyrene and anthracene were 6.450, 7.923, 8.233, 17.760, and 18.740 min, respectively, which differed from the peak order of standard HJ 478-2009 and HJ 647-2013. Conclusion When determining 16 kinds of polycyclic aromatic hydrocarbons simultaneously by HJ 478-2009, HJ 647-2013, HJ 784-2016, the peak sequence and time of polycyclic aromatic hydrocarbons should base on HJ 784-2016.