果蔬中甜蜜素残留快速检测试纸的研究

Study on rapid test paper for determination of Sodium Cyclamate in fruits and vegetables

课题研究快速检测果蔬中是否残留甜蜜素的试纸。从试纸显色剂种类、显色剂浓度、试纸材料、浸泡时间、干燥温度、干燥时间、保存条件等方面进行研究。确定试纸材料为普通滤纸;显色剂为0.8%磺胺(10%盐酸稀释):0.4%N-1-萘乙二胺盐酸盐(1:1);浸泡时间10min;试纸的干燥温度为50℃;干燥时间为40～45min;甜蜜素标准品检出限为0.01mg/mL;保存条件为常温密封保存。检测的6种果蔬样品中有3种检出甜蜜素残留,3种未检出。该检测试纸具有制作简单、成本低、体积小、携带方便、操作简单、检测快等特点,能对果蔬中甜蜜素残留进行快速定性检测。

The subject research was a rapid test paper for determination of sodium cyclamate residues in fruits and vegetables. The research was carried out from the aspcets of the type of test teagent chromogenic agents,the concentration of the chromogenic agents, the material of the test paper, the soaking time, the drying temperature, the drying time, and the preservation conditions. Make sure that the test paper material was ordinary filter paper;The chromogenic agents was 0.8% sulfanilamide (diluted with 10% hydrochloric acid) :0.4% N-1-naphthalene ethylenediamine hydrochloride (1:1);The soaking time of test paper was 10 min, the drying temperature was 50℃, the drying time was 40~45min;The detection limit of standard sodium cyclamate was 0.01mg/mL;The preservation conditions was sealed at room temperature. Three of the six fruit and vegetable samples tested detected cyclamate residues and three were not detected.The test paper has the characteristics of simple manufacture,low cost,small volume,convenient carrying,simple operation barrel and fast detection. It could quickly and qualitatively detect residues of sodium cyclamate in fruits and vegetables.