药食同源植物鼠曲草中6种黄酮类成分的含量测定

A Determination of 6 flavonoids in medicinal and edible homologous plants of Gnaphalium affine

建立了高效液相色谱法同时测定鼠曲草中6种黄酮类成分:槲皮素,木犀草素,芹菜素,山奈酚,金丝桃苷,木犀草素-4’-O-β-D-葡萄糖苷含量的方法。样品用80%甲醇超声提取,采用HPLC法检测,色谱柱:Agilent Eclipse XDB C18Column (250×4.6 mm I.D.,5μm);流动相:乙腈-0.1%甲酸梯度洗脱;流速:1.0 mL/min;柱温:30℃;检测波长:360 nm。结果表明,在一定的浓度范围内6种黄酮的浓度与峰面积线性关系良好,相关系数R^2为0. 9997～0. 9999,样品的加样回收率为95. 20%～108. 5%,RSD均≤2. 8%。本方法简便、快捷、精密度高、重现性好,可用于鼠曲草中黄酮类成分的含量测定。研究结果为明确鼠曲草作用的物质基础以及制定质量标准提供很好的依据。

sEstablishing a HPLC method for simultaneous determination of the content of 6 flavonoids: quercetin,chromatography luteolin, apigenin, kaempferol, Hyperoside in Gnaphalium affine. Samples were extracted by 80% methanol with ultrasonic. HPLC method was as follows: chromatographic column:Agilent Eclipse, XDB C18 Column (250 × 4.6 mm I.D., 5 μ m), mobile phase: gradient elution by acetonitrile -0.1% methanoic acid, flow rate: 1 mL/min, column temperature: 30 ℃, detection wavelength: 360 nm. The results showed that, the linear relationship between concentration and peak area of 6 flavonoids was good in certain concentration range, the correlation coefficient R^2 was 0.9997~ 0.9999, the recovery rate of sample was 95.20% ~ 108.5%, RSD ≤ 2.8%. The method is simple, fast,high precision, good reproducibility, can be used to determine the content of flavonoids in the Gnaphalium affine. The result of this study could provide a good basis on explicating the material basis of Gnaphalium affine and establish the quality standard.