制动致福利受损大鼠部分行为学改变及其体内NF-κB的变化

NF-κB Expression and Partial Behavioral Changes in Welfare Impaired Rats Caused by Restrain-stress

目的观察制动致福利受损SD大鼠部分行为学改变以及体内NF-κB的变化。方法选取体质量约200 g的雄性SD大鼠,使用管状容器给予束缚应激刺激,并通过旷场实验和O迷宫观测大鼠在第0、1、3、7、10、14天的行为学表现。测定了相应时期的NF-κB p65 mRNA转录水平和蛋白表达量。结果束缚第3天起,大鼠肝脏组织NF-κB p65 mRNA转录水平和蛋白表达均较束缚前显著增高(P<0.05),但此时尚未见行为学改变;束缚第7天起大鼠的活动兴趣与活动能力水平较束缚前显著下降(P<0.05),显示焦虑、恐惧状态。结论制动致福利受损大鼠肝细胞内NF-κB mRNA及蛋白表达的变化早于行为学的改变。

Objective To study the behavioral and NF-κB expression changes in welfare impaired rats caused by restrain-stress. Method Wrap restraint stress(WRS) were administrated by using tubular container within male SD rats(± 200 g). At time points of 0, 1, 3, 7, 10 and 14 days after WRS administration, open filed test and elevated o-maze test were employed to analyze the physical behavior changes of SD rats. NF-κB p65 mRNA transcription levels and protein expression in all SD rats were examined as well. Result Significant expression enhancements were observed both in NF-κB p65 mRNA transcription and NF-κB p65 protein expression at the 3 rd day after WRS administration compared with pre-procedure(P<0.05), but no behavior changes could be found. At the 7 th day after WRS administration, significant decline(P<0.05) in physical activity capacity and activity intension were noticed. All SD rats exhibited symptoms of depression and dread. Conclusion Changes in NF-κB mRNA and protein expression may prior to behavioral changes in welfare impaired rats.