油菜含油量相关基因Gpat、Lpat在不同肥密条件下表达规律研究

Study on Expression Patterns of Gpat and Lpat Related to Oil Content in Rape under Different Fertilizer and Density Conditions

油菜是我国第一大自产油料作物,具有重要的食用、饲用等价值。提高油菜籽含油量一直是育种家们不懈追求的目标之一,不同的栽培措施对油菜含油量有较大影响,为了研究不同施肥量、种植密度对油菜中控制含油量相关基因Gpat、Lpat表达量的影响,本研究采用qRT-PCR测定不同时期、不同肥密条件下Gpat、Lpat基因的表达量,并对其表达情况进行了分析。研究得知:Gpat、Lpat表达量变化规律是相同的,即:随着氮肥的增加而降低,随着密度的增加而升高,随着硼肥的增加而先升高后下降。此外,本研究在A1B3C1、A1B2C2及A2B3C2处理下可同时促进Gpat与Lpat的表达,是比较理想的肥密条件,可为提高油菜的含油量提供参考。

Rape is the largest self-produced oil crop in China, and it has important food and feeding value. Raising oil content of rapeseed is always one of the goals pursued by breeders. The cultivation measures have great influence on the rapeseed oil content. In order to study the effect of different amount of fertilizer and planting density on the expression of oil content related gene Gpat and Lpat in Brassica napus, the expression of these genes was measured by qR T-PCR at different periods and different density and fertilizer conditions and it was then analyzed. The results showed that the change patterns of Gpat and Lpat expression were the same, namely: the expression reduced with the increase of nitrogen, while it increased with the increase of the density and it increased firstly and then decreased with the increase of boron fertilizer. In addition, in this study, the treatment of A1 B3 C1, A1 B2 C2 and A2 B3 C2 can promote the expression of Gpat and Lpat at the same time, which might be an ideal condition of fertilizer and density and could provide reference for improving the oil content of rape.