黄连素对肥胖小鼠肠道菌群的影响及其机制

Effect of Berberine on Intestinal Flora of Obese Mice and Its Mechanism

为了探索黄连素对肥胖小鼠肠道菌群的影响和作用机制,本研究将40只雄性昆明种小鼠随机分为空白组、模型组、黄连素高剂量组、黄连素中剂量组和黄连素低剂量组,每组8只,除空白组外,其余4组给予高脂饮食,建立实验性肥胖小鼠模型。造模14 d后,全部给予正常饮食,黄连素高、中、低剂量组灌胃给予每天0.1～0.3 mL/10 g的药物干预,空白组和模型组给予等剂量的生理盐水,给药持续14 d。每周称量两次体质量,并分别于实验第0、14、28天从眼眶取血测定血脂和炎性因子的含量,收集小鼠粪便测定乳酸杆菌和双歧杆菌的数量。研究结果表明:空白组和模型组小鼠的体质量在第14天时有明显差异,造模成功;实验第28天,黄连素各给药组小鼠的体质量与模型组相比有显著性差异。模型组双歧杆菌、乳酸菌的数量和血脂水平与空白组比较有显著性差异,黄连素给药组能显著改善肥胖小鼠的血脂水平和双歧杆菌、乳酸杆菌的数量。本研究结果初步得出结论认为:黄连素改善肥胖小鼠的作用机制可能与脂质代谢、炎性反应和肠道微环境的改变密切相关。

In order to explore the effect and action mechanism of berberine on intestinal flora of obese mice, 40 male Kunming mice were randomly divided into the control group(CN), the control model group(CM), the berberine high-dose group(BH), the berberine medium dose group(BM), and the berberine low dose group(BL), with 8 mice in each group. In addition to the blank group, the other 4 groups were given high-fat diet to establish an experimental obese mouse model. After 14 days of modeling, normal diet was given to all, and 0.1~0.3 mL/10 g of drug intervention was given by the gastric lavage to the groups with high, medium and low doses of berberine, and isodose saline was given to the blank group and the model group for 14 days. The mass of the body was weighed twice a week, and the contents of blood lipids and inflammatory factors were measured in the orbital blood taken at day after 0, 14 and 28 days, respectively. The quantity of lactobacillus and Bifidobacterium was measured in mouse feces. The results showed that there was significant difference in body mass between the control group and the control model group at the 14 thday, which indicated successful molding. The results showed that on the 28 th day of the experiment, the body mass of mice given berberine was significantly different from that of the control model group. The number and blood lipid level of Bifidobacteria and lactobacillus in the control model group were significantly different from that in the control group. The berberine administration group could significantly improve the lipid level and the number of B ifidobacteria and lactobacillus in the obese mice. The resultspreliminarily concluded that the mechanism of berberine to improve obese mice might be closely related to lipid metabolism, inflammatory response and changes in intestinal microenvironment.