免疫性血小板减少症患者调节性B细胞变化与临床意义

Level of Regulatory B Cells in Patients with Immune Thrombocytopenia and Its Clinical Significance

目的:本研究旨在探讨调节B细胞(Breg)在免疫性血小板减少症(ITP)发病中的作用及其临床意义。方法:采用流式细胞术检测40例ITP患者(ITP组)的Breg、Th1、Th2、Th17以及Treg细胞的细胞量,应用AimPlex流式高通量技术检测IL-10、TGF-β、CD40以及CD40L的水平,选择20例健康正常人作为对照(对照组)。结果:与对照组相比较,ITP患者Breg细胞的细胞数量显著少于对照组(P <0.05),IL-10、TGF-β以及CD40L的水平亦显著低于对照组(P <0.05)。Th1细胞数量显著增多(P <0.05),而Th2、Th17以及Treg细胞量均显著减少(P <0.05)。结论:Breg细胞在ITP发病机制中可能具有重要作用。

Objective: To investigate the role of regulatory B cells(Breg) in pathogenesis of immune thrombocytopenia(ITP) and its clinical significance. Methods: A total of 40 ITP patients and 20 normal controls were enrolled in this study. The content of Breg, Th1, Th2, Th17 and Treg cells were detected by flow cytometry(FCM). The expression level of IL-10, TGF-β, CD40 and CD40 L was detected by AimPlex Flow High Throughput Screening Technology. Results: The of Breg cells in ITP patients was significantly lower than that in normal controls(P <0.05),the expression levels of IL-10, TGF-β and CD40 L in ITP patients were also significantly lower than those in normal controls(P <0.05). The contents of Th1 cells in ITP patients were significantly higher than that in normal controls(P <0.05), whereas the contents of Th2, Th17 and Treg cells in ITP patients were significantly lower than those in normal controls(P <0.05). Conclusion: The Breg cells may play an important role in the pathogenesis of ITP.