台湾省苹果上牛眼果腐病菌的截获鉴定

Isolation and identification of Neofabraea kienholzii on Malus pumila imported from Taiwan Province

为明确从宁波空港口岸入境旅客携带苹果上截获的1株明孢盘菌属菌株60017的分类地位,通过形态学特征观察、内转录间隔区(ITS)和β-微管蛋白基因(β-tubulin)序列比对分析以及致病性测定,对该菌株进行了种类鉴定。结果表明:菌株60017在PDA培养基上的菌落为圆形,边缘整齐,呈乳白色,气生菌丝较少,可产生大量分生孢子;基于ITS和β-tubulin序列构建的系统发育树中,菌株60017和苹果牛眼果腐病菌Neofabraea kienholzii处于同一分支;菌株60017接种苹果后,在接种部位能引起褐腐症状,与原发病症状一致。结合形态学特征、致病性测定结果以及分子鉴定结果,最终将该菌株鉴定为苹果牛眼果腐病菌N. kienholzii。

To identify the fungal strain 60017 isolated from the apple imported from Taiwan Province of China, the morphological observation, sequence analysis and pathogenicity test were carried out. The morphological results showed that the colony of the strain 60017 on PDA medium was round, with neat edges, milky white, less aerial hyphae, and could produce a large number of conidia. Its rDNA ITS and β-tubulin sequences were sequenced. Analysis of these sequences with the related sequences in NCBI showed that the strain 60017 clustered into Neofabraea kienholzii clade. The pathogenicity test showed that the strain 60017 could infect apple fruit with the symptoms of brown rot. These results indicated that the fungal strain 60017 was N. kienholzii.