四种菊酯类杀虫剂对枸杞蚜虫线粒体膜流动性的影响

Effects of four pyrethroids on mitochondrial membrane fluidity of Aphis sp.

为明确菊酯类杀虫剂对昆虫线粒体膜流动性的影响,以1,6-二苯基-1,3,5-己三烯(1,6-diphenyl-1,3,5-hexatriene,DPH)为荧光探剂,采用荧光偏振法研究了4种菊酯类杀虫剂对枸杞蚜虫Aphis sp.线粒体膜流动性的影响。结果表明,4种菊酯类杀虫剂对枸杞蚜虫线粒体膜流动性均有显著影响,其荧光偏振度显著增大,线粒体膜流动性显著减小。当药剂浓度为100μmol/L时,对照组枸杞蚜虫线粒体膜的荧光偏振度为0.239,高效氯氰菊酯的影响最大,线粒体膜的荧光偏振度为0.315,联苯菊酯的影响最小,线粒体膜的荧光偏振度为0.287。4种菊酯类杀虫剂对线粒体膜流动性的影响都存在一定的浓度效应,当药剂浓度在0～100μmol/L范围内,浓度越大影响越大。4种菊酯类杀虫剂在不同温度下对枸杞蚜虫线粒体膜流动性的影响不同,高效氯氰菊酯、联苯菊酯和甲氰菊酯对线粒体膜流动性的影响基本不受温度影响,而氯菊酯在高温条件下产生的影响大,在低温条件下产生的影响小,表现为明显的正温度系数。表明DPH可以作为荧光探剂研究枸杞蚜虫线粒体膜的流动性。

To determine the effects of pyrethroids, bifenthrin, β-cypermethrin, fenpropathrin and permethrin, on the fluidity of mitochondrial membrane in insects, four pyrethroids were studied by using 1,6- diphenyl-1,3,5-hexatriene (DPH) as a fluorescence probe in Aphis sp. The results showed that the fluidity of mitochondrial membrane could be affected by the four pyrethroids. The four pyrethroids increased the values of DPH polarization of mitochondrial membrane, while the fluidity of mitochondrial membrane decreased. When the concentration of pyrethroids was 100 μmol/L, the extent of effect caused by β-cypermethrin was the largest, with a DPH polarization value of 0.315 (the value of CK was 0.239). The smallest value (0.287) was detected in bifenthrin among the tested pyrethroids. In the range of 0-100 μmol/L, the effects increased with increasing concentrations of the four pyrethroids. The effects of pyrethroids on the fluidity of mitochondrial membrane were different at different temperatures. The effect caused by permethrin was greater at higher temperature than at lower temperature, showing a positive temperature coefficient to permethrin. There was no interaction between the effects of the other three pyrethroids on the fluidity of membrane and the temperature. The results indicated that DPH could be used as a fluorescence probe to study the fluidity of mitochondrial membrane of Aphis sp.