小鼠黑色素瘤功能异常CD8^+T细胞基因调控网络构建与分析

Construction and Analysis of Gene Regulatory Network in Dysfunctional CD8^+T Cells

疾病是全球范围内危害畜禽健康的主要问题,解析免疫系统调控相关基因成为当前抗病分子育种的研究热点。为研究小鼠黑色素瘤模型功能异常CD8^+T细胞对免疫抗体处理的应答机制,通过基因调控网络方法筛选功能异常CD8^+T细胞在Ig G和PD1抗体处理的特定基因、转录因子和细胞表面受体。结果发现对照组、PD1抗体与Ig G抗体处理组分别有28、17、33个特定表达基因。与对照组相比,Ig G和PD1处理组有2个转录因子Zfx、Zfhx3关闭表达,PD1处理组细胞表面受体Raet1b表达。Raet1b基因的表达可能使小鼠黑色素瘤功能异常CD8^+T细胞恢复功能,结果为抗病育种的相关基因研究提供理论基础。

Disease is a major threat to livestock and poultry health worldwide. Identifying key genes related to disease resistance has become a research hotpot in molecular breeding of disease resistance. To better understand the response mechanism of dysfunctional CD8^+T cell to immune therapy treatment,the gene regulatory network was constructed for the dysfunctional CD8^+T cells under PD1,Ig G antibody treatments. There were 28,17,33 genes being identified in control,Ig G and PD1 antibody treatment groups,respectively. Two transcription factors of Zfx and Zfhx3 expressed silencing under treatments of both Ig G and PD1. The surface receptor of Raet1 b expressed increasingly under treatment of PD1,which might recover dysfunctional CD8^+T cells. This research provided theoretical basis for gene exploring related to disease resistance breeding.