α-亚麻酸对TM3细胞活性及相关基因表达的影响

Effects of ALA on Mice TM3 Leydig Cell Viability and Relative Gene Expression

α-亚麻酸(ALA)是哺乳动物必需脂肪酸的重要成员,在机体生长和发育过程中起着重要作用。在雄性动物生殖系统,ALA通过代谢生成二十二碳六烯酸(DHA)和二十碳五烯酸(EPA),进而对生殖系统发挥调控功能。采用WST-1、JC-1以及RT-PCR等方法研究ALA对体外培养的小鼠睾丸间质细胞TM3细胞的活力及相关基因表达的影响。结果表明:培养体系中添加10,50,100,200,400μmol/L的ALA,处理24 h可以显著提高TM3细胞活力(P<0.05),并且呈剂量依赖性。与对照组相比,50μmol/L的ALA处理24 h可以促进TM3细胞增殖,提高线粒体膜电位,抑制凋亡相关基因Caspase-3表达(P<0.05),促进类固醇代谢相关基因StAR表达(P<0.05)。这说明在体外培养体系中添加ALA可以增加TM3细胞活性,促进细胞增殖和StAR基因表达,抑制Caspase-3基因表达。

As one of the essential fatty acids, α-linolenic acid(ALA) plays an important role in the growth and development of the body. In the male reproductive system, ALA can convert into docosahexaenoic acid and eicosapentaenoic acid, and contribute to male fertility. The present study was undertaken to elucidate direct ALA effects on mice TM3 Leydig cell viability and gene expression using WST-1, JC-1 and real time PCR techniques. The results showed that the addition of different concentrations of ALA(10, 50, 100, 200 and 400 μmol/L) in the culture system for 24 h significantly up-regulated TM3 cell viability in a dose-dependent manner(P<0.05). Meanwhile, the mitochondrial membrane potential was remarkably boosted after being treated with 50 μmol/L ALA for 24 h. ALA also inhibited apoptosis related gene Caspase-3 expression(P<0.05) and stimulated steroid metabolism related gene StAR expression(P<0.05). To conclude, the present study demonstrated that ALA increased mice TM3 Leydig cell viability and stimulated proliferation and StAR expression, however, inhibited Caspase-3 expression.