褪黑素对绒山羊精液冷冻保存效果的影响

Effect of melatonin on the cryopreservation of cashmere goat semen

为了研究褪黑素对绒山羊精液冷冻保存效果的影响,在冷冻稀释液中外源补充不同浓度(0.125,0.250,0.500,1.000 mg/mL)的褪黑素进行冷冻试验,冷冻解冻采用计算机辅助精液分析系统(CASAS)和流式细胞仪分别检测精子活率、质膜完整率、顶体完整率以及精子细胞中活性氧(ROS)的含量。结果表明:在冷冻稀释液中添加褪黑素能够显著降低精子细胞中ROS的含量,而且在冷冻稀释液中添加浓度为0.500 mg/mL的试验组绒山羊精子活率(53.3%)、质膜完整率(44.6%)、顶体完整率(77.9%)均显著高于空白对照组(47.8%、36.3%和53.2%)(P<0.05)。说明在绒山羊精液冷冻稀释液中添加0.500 mg/mL褪黑素能够显著提高绒山羊精子抗氧化能力和冷冻保存效果。

In this sdudy,different concentrations of melatonin ( 0. 125,0. 250,0. 500,1. 000 mg /mL) were supplemented in frozen diluent to investigate the effect of melatonin on the cryopreservation of cashmere goat’s semen. The analysis system ( CASAS) and flow cytometry were used to detect sperm motility,plasma membrane integrity,acrosome integrity,and reactive oxygen species ( ROS) in sperm cells after thawing. The results show that the addition of melatonin in the freeze-diluted solution significantly reduced the level of ROS in the sperm. The sperm viability ( 53. 3%) ,plasm integrity rate ( 44. 6%) ,intact acrosome rate ( 77. 9%) were significantly higher than that of the control group ( 47. 8%,36. 3% and 53. 2%,P<0. 05) .It indicats that the addition of 0. 500 mg /mL melatonin to the frozen dilution of cashmere goat semen can significantly improve the antioxidant capacity and cryopreservation effect of spermatozoa.