基于~1H-NMR代谢组学研究夏秋茶对肉鸡脂肪和氨基酸代谢的影响

The effect of tea powder on fat and amino acid metabolism in broilers based on ~1H-NMR metabolomics

通过饲养试验,采用核磁共振(~1H-NMR)代谢组学技术研究夏秋绿茶对肉鸡脂肪和氨基酸代谢的影响。将240只试验肉鸡分为4组,分别饲喂添加0.00、2.50、5.00和10.00g·kg^(-1)夏秋茶粉的玉米-豆粕型试验饲粮,采用核磁共振代谢组学技术评价其血清与氨基酸和脂肪代谢有关的代谢产物,同时测量了肉鸡生长性能、血清生化指标和抗氧化指标。结果表明,在肉鸡饲料中添加一定浓度的绿茶粉具有明显的代谢终产物调节氨基酸和脂肪代谢的代谢效应,通过增强甘氨酸/丝氨酸和苏氨酸代谢和丙氨酸、天冬氨酸和谷氨酸代谢,增强了肉鸡抗氧化能力。其血清核磁谱表明可将谷氨酸、谷氨酰胺和甘氨酸视为绿茶粉调节代谢的潜在生物标志物,同时饲粮中添加绿茶粉不影响肉鸡的肝肾功能,对肉鸡的生产性能又一定改善作用。

By way of feeding trials, this experiment was conducted to study the effects of supplemental Summer-autumn tea power on fat and amino acid metabolism in broilers by nuclear magnetic resonance(1H-NMR)-based metabolomics. Broilers were divided into four groups, fed with dietary containing of 0.00, 2.50, 5.00 and 10.00 g·kg^-1 tea powder based on corn-soybean meal test diets, respectively. Serum samples were analyzed by ^1H-NMR metabolomics, to identify metabolites related to amino acid and fat metabolism. Growth performance, serum biochemical index and antioxidant index were detected among these groups, simultaneously. The results showed that a certain level of green tea powder supplementary has considerable metabolites to adjust amino acids and fat metabolism. Enhancing and broilers antioxidant capacity was enhanceed by reinforcing glycine, serine and threonine metabolism and alanine, aspartate and glutamate metabolism. Its serum ^1H-NMR spectroscopy showed that glutamic acid, glutamine and glycine can be regarded as potential biomarkers for adjusting the metabolism of broilers fed with Summer-autumn tea power. In addition, Summer-autumn tea power supplementary has not effect on liver and kidney function and certainly improves the performance of broiler.