幽门螺杆菌感染对肝癌组织增殖、侵袭和转移相关指标的影响

Effect of Helicobacter Pylori ( HP) Infection on Proliferation, Invasion and Metastasis of Hepatocellular Carcinoma(HCC)

【目的】 探讨幽门螺杆菌(Helicobacter pylori,HP)感染对肝癌组织增殖、侵袭和转移相关指标的影响。【方法】 选取2015年3月至2017年3月本院收治的肝癌患者癌组织标本80例和距离癌组织边缘≥5 cm的正常肝组织80例作为研究样本,每份样本分为两份,一份立即进行HP检测,另一份通过免疫组化实验检测增殖相关因子[B淋巴细胞瘤2基因(Bcl-2)、凋亡抑制基因(Survivin)]和侵袭转移相关因子[转凝蛋白(Transgelin)、α2整合素(Integrin α2)、细胞黏附因子-1(ICAM-1)、基质金属蛋白酶2(MMP-2)、血管内皮生长因子(VEGF)]表达情况。【结果】 肝癌组织中HP阳性率显著高于正常肝组织,肝癌组织中增殖和侵袭转移相关因子表达阳性率显著高于正常肝组织(P<0.05)。HP感染者肝癌组织中Bcl-2和Survivin阳性率分别是92.73%和94.55%,显著高于非HP感染者(P<0.05);HP感染者肝癌组织中Transgelin、Integrin α2、ICAM-1、MMP-2、VEGF阳性率分别是92.73%、96.36%、94.55%、92.73%和96.36%,显著高于非HP感染者(P<0.05)。【结论】 HP参与肝癌组织的发生发展,可促进肝癌组织增殖、侵袭和转移。

【Objective】 To investigate the effect of Helicobacter pylori (HP) infection on proliferation, invasion and metastasis of hepatocellular carcinoma (HCC).【Methods】 Eighty liver cancer specimens from March 2015 to March 2017 and 80 normal liver specimens (> 5 cm from the edge of the cancer tissue) were selected as study samples. Each sample was divided into two parts, one was detected for HP immediately, the other was detected by immunohistochemistry for the expression of proliferation-related factor [B lymphocytoma 2 gene (Bcl-2), apoptosis suppressor gene (Survivin)] and invasion and metastasis related factor [transagglutinin (Transgelin),Integrinα2, cell adhesion factor (ICAM-1)], Matrix metalloproteinase 2 (MMP-2) and vascular endothelial growth factor (VEGF).【Results】 The positive rate of HP in HCC tissues was significantly higher than that in normal liver tissues, and the positive rate of proliferation, invasion and metastasis related factors in HCC tissues was significantly higher than that in normal liver tissues (P<0.05). The positive rates of Bcl-2 and Survivin were 92.73% and 94.55% in HP-infected liver cancer tissues, respectively, which were significantly higher than those in non-HP-infected liver cancer tissues (P<0.05);the positive rates of Transgelin, Integrin alpha 2, ICAM-1, MMP-2 and VEGF in HP-infected liver cancer tissues were 92.73%, 96.36%, 94.55%, 92.73% and 96.36%, respectively, which were significantly higher than those in non-HP-infected liver cancer tissues (P<0.05). 【Conclusion】 HP is involved in the occurrence and development of HCC, and can promote the proliferation, invasion and metastasis of HCC.