Yes相关蛋白1和内质网蛋白29在结直肠癌组织的表达及临床意义

Expression of Yes-associated protein 1, endoplasmic reticulum protein 29 in colorectal carcinoma and their clinical significance

目的 探讨检测Yes相关蛋白1(YAP1)和内质网蛋白29(ERp29)在结直肠癌组织中的表达水平,以及两者与结直肠癌临床病理因素的关系。方法 应用免疫组织化学法检测85例结直肠癌组织和癌旁组织中YAP1和ERp29表达,分析两者与结直肠癌各临床病理因素的关系。结果 YAP1在结直肠癌组织中的阳性表达率为61.18%(52/85),明显高于癌旁组织中YAP1阳性表达率17.65%(15/85),两者差异有统计学意义(t=43.731,P<0.01),YAP1表达水平与与结直肠癌TNM分期、淋巴结转移呈明显相关(分别为t=6.632、5.273,P<0.05);ERp29在结直肠癌组织中的阳性表达率为42.35%(36/85),明显低高于癌旁组织中ERp29阳性表达率95.29%(81/85),两者差异有统计学意义(t=55.515,P<0.01),ERp29表达水平与结直肠癌TNM分期、浸润深度、淋巴结转移呈明显相关(分别为t=5.058、5.472、5.908,P<0.05)。结论 YAP1和ERp29的异常表达与结直肠癌的发生发展有关,检测YAP1和ERp29表达水平有助于判断结直肠癌浸润转移潜能及评估结直肠癌患者的预后。

Objective To investigate the expression of Yes-associated protein 1 (Yap1), endoplasmic reticulum protein 29 (ERp29) in colorectal carcinoma and their relationship with clinicopathological features.Methods The expression levels of YAP1 and ERp29 in 85 cases of colorectal carcinoma tissues and adjacent colorectal tissues were detected by using immunohistochemical method.Results The positive rate of YAP1 expression in colorectal carcinoma tissues (61.18%, 52/85) was significantly higher than that in non-cancerous adjacent colorectal tissues (17.65%, 15/85) (t=43.731, P<0.01), the expressions of YAP1 were related colorectal carcinoma to TNM stage, lymph node metastasis (Respectively, t=6.632, 5.273, P<0.05);the positive rate of ERp29 expression in colorectal carcinoma tissues (42.35%, 36/85) was significantly lower than that in non-cancerous adjacent colorectal tissues (95.29%, 81/85) (t=55.515, P<0.01), the expressions of ERp29 were related colorectal carcinoma to TNM stage, serosa invasion, lymph node metastasis (Respectively, t=5.058, 5.472, 5.908, P<0.05).Conclusion YAP1 and ERp29 were abnormal experssion in colorectal carcinoma, Detecting the expressions of YAP1 and ERp29 simultaneously in colorectal carcinoma have certain reference value for judging invasion and metastasis potential and evaluating prognosis of patients with colorectal carcinoma.