长链非编码RNA SUMO1P3对胃癌细胞增殖及凋亡的影响

Effects of long noncoding RNA small ubiquitin-like modifier 1 pseudogene 3 on proliferation and apoptosis of gastric cancer cells

目的 观察长链非编码RNA(lncRNA)SUMO1P3在胃癌组织中的表达水平及其对胃癌细胞增殖及凋亡的影响。方法 采用实时荧光定量反转录聚合酶链反应(RT-qPCR)检测SUMO1P3在63例胃癌及癌旁组织和胃癌细胞株(MGC803、AGS、BSG823、SGC7901)及正常人胃黏膜上皮细胞株(GES-1)中的相对表达量。将胃癌细胞株MGC803分为两组,小干扰RNA组(si-SUMO1P3组)和阴性对照组(si-Ctrl组),分别以细胞计数试剂盒(CCK-8)法、克隆形成实验、划痕愈合实验及流式细胞术检测干扰SUMO1P3表达对胃癌细胞增殖能力、克隆形成能力、细胞迁移能力及凋亡的影响。结果 胃癌组织中SUMO1P3相对表达水平(2.37±0.59)显著高于癌旁组织(0.91±0.28,t=17.740,P<0.01),且SUMO1P3在胃癌细胞株MGC803、AGS、BSG823、SGC7901中的表达量明显高于GES-1(P<0.05)。小干扰RNA(siRNA)能够明显抑制MGC803细胞中lncRNA SUMO1P3的表达。CCK-8实验结果显示,72 h后si-SUMO1P3组细胞吸光度值(2.22±0.14)低于si-Ctrl组(3.12±0.13,t=4.730,P<0.01)。克隆形成实验结果显示,si-SUMO1P3组克隆形成率(26.2±5.5)%低于si-Ctrl组(47.3±6.9)%(t=4.140,P<0.05)。划痕愈合实验结果显示,24 h后si-SUMO1P3组细胞迁移率(21.7±4.1)%低于si-Ctrl组(35.3±5.8)%(t=3.320,P<0.05)。细胞凋亡结果表明,si-SUMO1P3组细胞凋亡率(16.4±2.3)%高于si-Ctrl组(7.3±1.6)%(t=5.630,P<0.01)。结论 lncRNA SUMO1P3在胃癌组织中高表达,干扰SUMO1P3表达可抑制胃癌细胞增殖并诱导细胞凋亡。

Objective To investigate the expression level of long noncoding RNA (lncRNA) small ubiquitin-like modifier 1 pseudogene 3(SUMO1P3) in gastric cancer tissues and the its effects on proliferation and apoptosis of gastric cancer cells.Methods The expression level of SUMO1P3 in 63 clinical samples of gastric cancer tissues and adjacent normal tissues, and gastric cancer cell lines (MGC803, AGS, BSG823, SGC7901) and normal gastric cell line (GES-1) were measured by real-time quantitative reverse-transcriptase polymerase chain reaction (RT-qPCR). The MGC803 cells were divided into two groups: the small interfering RNA group (si-SUMO1P3) and the negative control group (si-Ctrl). The influence of SUMO1P3 interference on the capacities of cell proliferation, colony formation, cell migration and cell apoptosis were evaluated with cell counting kit-8 (CCK-8), colony formation assay, scratch healing experiment and flow cytometry, respectively.Results The relative expression level of SUMO1P3 was significantly higher in gastric cancer tissues (2.37±0.59) than that in adjacent normal tissues (0.91±0.28) (t=17.740, P<0.01), and SUMO1P3 was overexpressed in gastric cancer cell lines MGC803, AGS, BSG823, SGC7901 compared with GSE-1 (P<0.05). The expression of lncRNA SUMO1P3 in MGC803 cells was significantly repressed by small interfering RNA (siRNA). The results of CCK-8 assay showed that the absorbance value of si-SUMO1P3 group (2.22±0.14) was significantly lower than that of si-Ctrl group (3.12±0.13, t=4.730, P<0.01) after culturing 72 h. The colony formation results revealed that the colony forming ability of si-SUMO1P3 group (26.2±5.5)% was significantly impaired compared with si-Ctrl group (47.3±6.9)% (t=4.140, P<0.05). The scratch healing experiment indicated that the cell migration rate of si-SUMO1P3 group (21.7±4.1)% was significantly lower than that of si-Ctrl group (35.3±5.8)% (t=3.320, P<0.05) after incubating 24 h. Flow cytometry results suggested that cell apoptosis of si-SUMO1P3 group (16.4±2.3)% was significantly increased compared with si-Ctrl group (7.3±1.6)% (t=5.630, P<0.01).Conclusion LncRNA SUMO1P3 was highly expressed in gastric cancer tissues. Interfering the expression of SUMO1P3 could inhibit the proliferation and induce cell apoptosis of gastric cancer cells.