超声波辅助提取叶下珠总生物碱工艺的研究

Ultrasonic-assisted extraction of total alkaloids from Phyllanthus urinaria Linn

目的:超声波辅助酸液热提法提取叶下珠总生物碱,并优化其提取工艺。方法:采用单因素与正交设计L_9(3~4),以总生物碱的提取量为考察指标,对超声波提取时间、酸液pH值、水浴时间、水浴温度等因素进行考察。结果:影响叶下珠总生物碱提取量的各因素程度依次为水浴温度(C)>酸液pH值(B)>超声时间(A)>水浴时间(D),其最佳条件为A_1B_1C_3B_2,即超声时间为20 min,酸液pH值为3,水浴时间为2 h,水浴温度为70℃,在此工艺条件下,叶下珠总生物碱提取量为0.533 mg/g。

Objective:To optimize extraction process of alkaloid from Phyllanthus urinaria Linn. Methods:To optimize the extraction process by using single-factor test and orthogonal test L9(34).,Results:Factors affecting the yield of total alkaloids were temperature of water bath(C) >acid pH(B)>extraction time of ultrasonic(A) >time of water bath(D). The optimal extraction condition was A1B1C3D2,which were extract time of ultrasonic for 20 min with acid pH of 3,water bath treatment time of 1.5 h and temperature of 70 ℃. The yield of total alkaloids from Phyllanthus urinaria Linn under this condition was 0.533 mg/g.