NGF对坐骨神经损伤后腰髓与损伤神经MBP含量变化的影响

Effect of NGF on the changes of MBP in lumber spinal cord and injured sciatic nerve

目的 探讨大鼠周围神经损伤后相应神经与脊髓组织髓鞘碱性蛋白 (Myelinbasicprotein ,MBP)含量变化及神经生长因子 (NGF)的影响。方法 Wistar大鼠行单侧坐骨神经切断 ,断端采用硅胶管桥接 ,管内注入NGF ,应用酶联免疫吸附法测定腰段脊髓和损伤坐骨神经组织MBP含量的变化 ,实验分为Ⅰ组 :生理盐水对照 ;Ⅱ组 :硅胶管内给NGF ;Ⅲ组 :硅胶管内给NGF +每日肌肉注射NGF(5 0 0ng/kg连续 2周 )。结果 治疗组之间比较无统计学意义 ;治疗组与对照组相比较有非常显著性差异 (P <0 0 1) ;治疗组2 4h、2周时MBP含量较伤前显著升高 (P <0 0 1) ,4周恢复到伤前水平。结论 NGF治疗能减少MBP含量 。

Objective To investigate the effect of nerve growth factor (NGF) on the content changes of myelin basic protein (MBP) in lumber spinal cord and injured sciatic nerve of Wistar rats. Methods Wistar rats was divided into one control grop and two experimental groups in which rats were given NGF. Content changes of MBP were measured by ELISA. Results No significant changes was observed between the experimental groups; and there existal significant difference between the experimental groups and the control group ( P <0.01); the content of MBP at 24h and 2 weeks after injury increased significantly ( P <0.01);and the content of MBP recovered 4 weeks after injury. Conclusion Treatment of NGF can reduce the content of MBP.