5-氟脲嘧啶及丝裂霉素C对人胃癌细胞株SGC-7901及BGC-823的相互作用的体外观察

The interaction between 5-fluorouracil and mitomycin C on human gastric carcinoma cell line SGC-7901 and BGC-823

目的 :体外观察 5 -氟脲嘧啶及丝裂霉素C对人胃癌细胞株 (SGC - 790 1及BGC - 82 3)的相互作用。方法 :采用MTT法利用中效原理判定两药合用的效果。结果 :两种药单独应用时随着药物剂量增加 ,其效应也增加 ;两药大剂量 (大于中效剂量 )合用时是协同作用 (CI<1 ) ,小剂量 (小于中效剂量 )合用时是拮抗作用 (CI >1 ) ;两药给药时间次序不同不会影响效应大小。结论 :上述两种药合用大剂量是协同 ,而小剂量则拮抗 。

AIM: To observe effects of 5-fluorouracil (5-FU) and mitomycin C (MMC) on human gastric carcinoma cell lines SGC-7901 and BGC-823. METHODS: The MTT assay and median-effect principle were used. RESULTS: The effects of the inividual drugs were enhanced as drug concentration increased. The interaction of the two drugs was synergistic at higher concentration (CI<1) and antagonistic at lower concentration(CI>1). The sequence of administration did not influence the effects. CONCLUSION: The interaction of the two drugs was cooperated at higher concentration (CI<1) and antagonistic at lower concentration (CI>1).