急性肺损伤大鼠多形核白细胞L选择素表达的变化及其意义

Changes of L-selectin expression on peripheral blood polymorphonuclear leukocytes and their significance in rats with acute lung injury

目的 :探讨急性肺损伤 (ALI)大鼠循环血多形核白细胞 (PMN)表面L选择素 (L -selectin)表达的变化及其在ALI发病中的作用。方法 :静注内毒素 (ET)复制大鼠ALI模型 ,循环血PMNL选择素表达用免疫荧光间接法及流式细胞仪进行检测。结果 :L选择素荧光信号集中分布于PMN细胞膜表面 ,对照组PMNL选择素表达最强 ;ET致伤后 5min大鼠PMN表面L选择素表达明显低于对照组 (P <0 0 5) ,ET致伤后 1 5 - 30minL选择素表达最低 (P <0 0 1 ) ,伤后 60min时PMN表面L选择素表达仍明显低于对照组 (P <0 0 1 )。结论 :正常状态下L选择素在PMN表面呈结构性表达 ,ET致伤后PMN表面的L选择素蛋白表达迅速减少 ,伤后 1 5min时最低。L选择素可能在ALI的发生发展过程中起一定的作用。

AIM: To explore the changes of L-selectin expression on peripheral blood polymorphonuclear leukocytes (PMNs) and their significance in rats with acute lung injury (ALI). METHODS: ALI model in rat was established by intravonous injection of E. coli endotoxin (ET). The expression of L-selectin on peripheral blood PMNs was measured by immunofluorescence and flow cytometry.RESULTS: The contribution of L-selectin fluorescence signal was on the surface of PMNs membrane. The expression of L-selectin on poeripheral blood PMN was significantly lower at 5 min after injection of ET and the lowest during 15 min to 30 min, then gradually increased, but the expression of L-selectin on PMN was lower at 60 min after injection of ET than that of control animal.CONCLUSION: In physiological state, L-selectin were expressed on the surface of PMN membrane. The protein expression of L-selectin on PMNs reduced rapidly after injection of ET and the lowest at 15 min, then gradually increased. L-selectin may play a role in the development of ALI.