骨关节炎软骨基质蛋白多糖变化的实验研究

Changes of proteoglycan in articular cartilage matrix of osteoarthritis in rabbits

目的 观察骨关节炎 (OA)不同时期关节软骨基质蛋白多糖的变化 ,探索与软骨基质代谢相关的OA的发病机制。方法 以家兔制作OA动物模型 ,在病变的早、中、晚期获取关节软骨 ,提取葡糖氨基聚糖 (GAG) ,用生化和放射免疫技术测定其中不同种类GAG及其功能基团的含量。结果 在病变的不同时期 ,GAG的总量、透明质酸的含量和其中所含的硫酸基团的含量均减少 ,而硫酸软骨素与硫酸角质素的比例增高。这些改变均随病变的进展而渐不明显。结论 OA时 ,软骨基质蛋白多糖各组分和功能基团均发生了有意义的变化 。

Objective To observe the changes of proteoglycan (PG) of articular cartilage matrix in osteoarthritis (OA) in different stages and to research the OA pathogenesis relative to cartilage matrix metabolism.Methods The rabbit model of OA was made for getting the articular cartilage in OA early,middle and late stages.Glycosaminoglycan (GAG) was extracted from cartilage,the quantity of different kinds of GAG and its function groups were detected with biochemical and radioimmunologic techniques.Results The quantity of total GAG,hayluronic acid and SO 2- 4 were all declined.These changes gradually diminished by the disease development but the CS/KS increased.Conclusion The contents of total PG,various PG composition and its function groups of articular cartilige matrix all have significant changes in OA different stages.These changes will aid to clarify the mechanism of OA formation.