口腔癌VEGF-C mRNA表达和血管及淋巴管生成与淋巴道转移关系的研究

Relationship among Expression of Vascular Endothelial Growth Factor-C(VEGF-C),Angiogenesis,Lymphangiogenesis,and Lymphatic Metastasis in Oral Cancer

背景与目的:VEGF-C与癌周的淋巴管血管生成以及肿瘤的淋巴道转移可能有密切的关系,本研究探讨口腔鳞癌组织血管、淋巴管密度与VEGF-CmRNA表达及淋巴道转移的关系。方法:VEGF-C逆转录PCR(RT-PCR),血管、淋巴管酶组化染色、光镜及图像分析观察血管、淋巴管总体面数密度(TNa)。结果:VEGF-CmRNA表达阳性的淋巴管TNa(26.42±5.85)明显高于阴性淋巴管TNa(17.34±6.48)(P<0.01);VEGF-CmRNA表达阳性的血管TNa(35.16±15.55)略高于阴性的血管TNa(33.49±13.73)(P>0.05)。淋巴结转移组血管TNa(44.19±14.29)比无淋巴结转移组TNa(30.61±11.82)增加(P<0.01)、淋巴结转移组淋巴管TNa(30.67±5.76)比无淋巴结转移组TNa(21.94±5.84)增加(P<0.01)。结论:VEGF-C主要介导了癌周淋巴管生成,对血管生成有一定影响;血管、淋巴管密度的同时增加可能与VEGF、VEGF-C及其受体的协同表达有一定关系。

Background &Objective:There may be a close relationship amo ng the vascular endothelial growth factor-C(VEGF-C)expression,peritumour lymphatic v essels,and lymph node metastasis.The purpose of this study was to compare the relationship among VEGF-C mRNA expression,density of lymphatic and blood vessels,and lymphati c metastasis in oral squamous carcinoma.Methods:VEGF-C mRNA was determined by RT-PCR.Lymph atic and blood vessel differential stain was determined b y enzyme-histochemical technique.Automated image analysis quantification was applied to determine the number of total lymp hatic and blood vessels in unit area(TNa ).Results:The peri-tumor lymphatic TNa of oral carcinoma was significantly higher in VEGF-C-positive group thanthose in VEGF-C-negative group(26.42±5.85:17.34±6.48)(P<0.01),but the blood TNa was a few higher(35.16±15.55:33.49±13.73)(P>0.05).Lymphatic TNa (30.67±5.76:21.94±5.84)(P <0.01)and blood TNa (44.19±14.29:30.61±11.82)(P<0.01)were significantly higher in lymph n ode metastasis group than those in no-metastasis group.Conclusion:VEGF-C mainly promotes peri-tumor l ymphangiogenesis and has a little effect on angiogenesis.Simultaneous increase of lymphatic and blood vessels may be related to the synergic expressio n of VEGF,VEGF-C and their receptors.