植物多糖增强肿瘤杀伤效应细胞的增殖活性和细胞毒活性

Enhancement of proliferaton and tumoricidal activities of T-AK cells by plant polysaccharides

目的 观察红景天、红芪、地黄、枸杞子、沙参和芦荟的多糖成分对 T- AK细胞增殖活性和杀瘤活性的增强作用。方法 用抗 CD3单抗 ( CD3Mc Ab)和 r IL - 2激活诱生 T- AK细胞 ,观察 6种植物多糖在诱生、扩增和杀瘤过程中对 T- AK细胞增殖活性、IL- 2 R表达水平以及杀伤 Raji细胞和 L1 2 1 0 细胞活性的影响。结果 6种植物多糖( 1～ 10 0 μg/ m L)单独对 T- AK细胞的增殖无明显作用 ,但在 r IL- 2和 CD3Mc Ab存在下 ,可不同程度地增强 T-AK细胞的增殖能力和杀瘤活性 ,并增加 T- AK细胞 IL - 2 R的表达。结论 红景天、红芪、地黄、枸杞子、沙参和芦荟的多糖成分增强 T- AK细胞的增殖和杀伤活性 ,其作用效应与其促进 IL- 2

Object To study the augmentation of the T activated killer (T AK) cell proliferating and tumor killing activities of the polysaccharide fraction from Rhodiola kirilowii (Regel) Regel, Lycium barbarum L., Hedysarum polybotrys Hand. Mazz., Glehnia littoralis F. Schmidt ex Miq., Rehmannia glutinosa (Gaert.) Libosch. ex Fisch. et Mey. and Aloe barbadensis Mill. in vitro. Methods The T AK cells were induced by anti CD 3 antibody (CD 3McAb) and rIL 2 from human peripheral blood mononuclear cells (PBMC). The effects of the above six plant polysaccharides (1～100 μg/mL) on the proliferation of T AK cell, the cytotoxicity to Raji cells and L 1210 cells, and the IL 2 receptor (IL 2R) expression in T AK cells were determined. Results The six polysaccharides alone had no obvious effect on the proliferation of T AK cells. In the presence of rIL 2 and CD 3McAb, they could reinforce the proliferation of T AK cells and its tumor killing activities against Raji cells and L 1210 cells at a different extent, and a 21%～68% increase of IL 2R expression in T AK cells was observed. Conclusion The plant polysaccharides significantly enhance the proliferation and the tumoricidal activities of T AK cells and the enhancing actions related to the increase of IL 2R expression.