野生型PTEN转染对胶质母细胞瘤基因表达的影响

The effects of wild-type PTEN transfection on gene expressions of glioblastomas

目的 研究野生型PTEN对胶质母细胞瘤基因表达的影响及意义。方法 用表达野生型PTEN的载体转染PTEN失活的胶质母细胞瘤细胞系U87MG ,筛选出稳定表达野生型PTEN的细胞克隆 ,然后用cDNA微阵列技术找出野生型PTEN转染后mRNA表达改变的基因。结果 转染的野生型PTEN可以明显抑制U87MG肿瘤细胞的增殖。用cDNA微阵列技术分析后 ,共有 89个cDNA克隆在PTEN转染细胞与对照组细胞之间差异有显著性 (P <0 .0 1)。其中 ,76个为已知基因 ,包括胶质纤维酸性蛋白、p2 1/WAF1、转化生长因子β诱导的早期蛋白、DNA碎裂因子 4 5等 ;13个为未知基因。 结论 野生型PTEN可以影响多种基因的表达 ,从而调控胶质母细胞瘤细胞的增殖、分化。

Objective To study the effects of wild type PTEN on gene expressions of glioblastomas. Methods Glioblastoma U87MG cells, which express inactivated PTEN, were transfected with wild type PTEN constructs and stable transfected clones were selected. Then, cDNA microarray analyses were used to identify differentially expressed genes in wild type PTEN transfected cells and control cells. Results Transfected wild type PTEN inhibited the proliferation of U87MG. By cDNA microarray analyses, 89 cDNA clones were identified, which were differentially expressed in wild type PTEN transfected cells and control cells. Among these genes, 13 genes were unknown and 76 genes were known genes, including glial fibrillary acidic protein, p21/WAF1, human TGF β inducible early protein, human DNA fragmentation factor 45 etc. Conclusion Wild type PTEN can affect the expressions of multiple genes, by which it regulates the proliferation, differentiation and apoptosis of glioblastomas.