支气管哮喘胸导管引流液中IL-10、IL-12和T细胞亚群检测及意义

Study on IL - 10、IL - 12 and T Lymphocyte Subset of Lymph in Thoracic Duct in Bronchial Asthma

目的:探讨支气管哮喘淋巴液和外周血IL-10、IL-12、T细胞亚群水平,及胸导管引流（TDD）的影响。方法:采用酶联免疫吸附试验（ELISA）检测31例中、重度哮喘病人TDD治疗0d、5d淋巴液和外周血的IL-10、IL-12及T细胞亚群。结果:哮喘淋巴液中IL-10明显高于正常对照组血浆水平,而IL-12则明显降低;TDD治疗5天,哮喘淋巴液和血浆中IL-10均明显下降,IL-12则显著升高达正常血浆水平。哮喘淋巴液中CD3、CD8百分率明显低于正常人外周血水平,而CD4/CD8比值、B细胞百分率则显著增高;哮喘淋巴液中CD3百分率、CD4/CD8比值显著高于其自身外周血水平;TDD治疗5天,哮喘淋巴液中CD4/CD8比值下降,同时外周血CD3、CD8百分率明显升高,CD4/CD8比值、B细胞百分率下降。结论:哮喘淋巴液中存在IL-10和IL-12产生或释放失调,以及T细胞亚群失衡。TDD可能通过引流部分细胞因子和T细胞,调整和促进细胞因子和T细胞亚群恢复正常。

: To study the level of lymph and plasma IL - 10、11 - 12 and T lymphocyte subset and the effect of thoracic duct drainage (TDD) in bronichial asthma. Methods: IL-10、IL - 12 in lymph(0 day、5 day) and plasma(0 day> 5 day)were measured by enzyme - linked immunosorbent assay (ELISA)and T lymphocyte subset were also detected in 31 cases with middle and serious asthma of performing TDD. Results: Level of lymph IL - 10 in asthma were higher than that in the plasma of controls,but lymph IL- 12 was lower than that in controls. In TDD 5 day,level of IL- 10 in lymph and plasma were significantly decreased but IL - 12 was raised to the plasma level of controls. The percentage of CD3 and CD8 in lymph asthma were lower than that in the healthy peripheral blood, but rate of CD4/CD8 and percentage of B lymphocyte in lymph were higher than that in healthy peripheral blood. The percentage of CD3 and rate of CD4/CD8 in lymph were high to itself level of peripheral blood in asthma. In TDD 5 day, the lower CD4/CD8 rate in lymph, increase of CD3 and CD8 percentage, and lower of CD4/CD8 rate and percentage of B lymphocyte in peripheral blood were detected in asthma. Conclusion: There were abnormal levels of IL- 10 and IL-12 and imbalance of T lymphocyte subsets in lymph of asthma. TDD might regulate CK and T lymphocyte subsets to normal by draining partly cytokines and T lymphocytes in asthma.