柯萨奇B_3病毒吸入致新生鼠持续气道高反应性的观察

Persistent airway hyperreactivity induced by inhaling coxsackie virus B_3 in neonatal rats

目的 :观察新生鼠呼吸道吸入柯萨奇 B3病毒 (CVB3)后气道反应性的变化特征 ,探讨病毒感染与气道高反应性(AHR)之间的关系。 方法 :建立新生 SD大鼠病毒感染模型 ,出生 5 d新生鼠超声雾化吸入 CVB3,接种后 10 d取眼球血用EL ISA法检测 CVB3- Ig M,6～ 8周用 EJD- 型多道生物信号分析系统测气道内压。结果 :病毒组新生鼠血清中 CVB3- Ig M的D值均在对照组 x+2 s以上 ;接种后 6～ 8周病毒组气道内压较对照组显著升高 (P<0 .0 1)。 结论 :新生鼠吸入 CVB3感染模型成立。持续的气道高反应可能与新生鼠吸入

Objective:To observe the changes of airway reactivity after inhalation of coxsackie virus B3,and to study the relationship between airway hyperreactivity and viral infection.Methods:Five- day- old neonatal SD rats inhaled CVB3by ultrasonic brume were taken as airway virus infection m odel.Specific Ig M to CVB3in sera was detected by EL ISA ,and the airway inner pressure was measured by EJD- Multi- channel Recorder.Results:The sera from experimental group had higher optical density of CVB3- Ig M by EL ISA 10 d post- inoculation compared with that of the control group.The airway resistance response was significantly increased 6 - 8weeks after virus challenge compared with those of control anim als. Conclusion:The airway hyperreactivity response of experimental rats m ay correlate with the inflam mation in the airway induced by CVB3airway inhaling. [