人多种组织中线粒体DNA存在13.1kb片段缺失突变

A 13.1 kb mitochondrial DNA deletion mutation exist in various human tissues

目的 :在人外周血细胞中筛选到新的线粒体 DNA大片段缺失突变后 ,进一步探讨该缺失在稳定组织内的分布。 方法 :以人外周血细胞及人体多种组织 DNA为模板 ,进行 PCR扩增 ,筛选该突变。结果 :在人外周血细胞及多种稳定组织中发现 13.1kb缺失突变的存在。 结论 :这种广泛分布于健康人多种组织中的线粒体

Objective:To study a newly- found large scale m itochondrial DNA deletion m utation in human tissues. Methods:PCR was carried out using DNA extracted from human peripheral blood cells and various tissues to screen the deletion. Results:The 13.1kb m t DNA deletion m utation was found in hum an peripheral blood cells and several hum an issues. Conclusion:This deletion m utation is found in several hum an issues in healthy people and it m ay be related with aging. [