海洋软体动物蓝斑背肛海兔抗肿瘤活性成分研究(Ⅱ)

Studies on antineoplastic constituents from marine mollusc sea hare (Ⅱ)

目的 :研究蓝斑背肛海兔 N otarchus leachii freeri的抗癌活性成分。方法 :利用溶媒萃取和多种层析手段 ,以稻瘟霉模型监测跟踪 ,对海兔中抗癌活性成分进行追踪分离 ,应用波谱技术鉴定化合物的结构。 结果 :得到 2个活性化合物 :3β-羟基 - 5α,6α-环氧 - 8-胆甾烯 - 7-酮 ( )和 3β,7α-二羟基 - 5α,6α-环氧 - 8( 14 ) -胆甾烯 ( )。 结论 :化合物 为新天然产物 , 为首次从蓝斑背肛海兔中得到 。

Objective:To investigate the antineoplastic constituents of sea hare Notarchus leachii freeri .Methods:Antineoplastic compounds were screened from sea hare by bioassay guided method with conidia of Pyricularia oryzae ,and by a combination of partition and purification with suitable solvent and multiple column chromatographies.Structures of active compounds were determined on the basis of spectral analysis.Results:Two active compounds were screened and the structures were elucidated as 3β hydroxy 5α,6α epoxycholesta 8 ene 7 one (Ⅰ) and 3β,7α dihydroxy 5α,6α epoxycholesta 8 (14) ene (Ⅱ).Conclusion:Compound Ⅰ is a new natural product,and compound Ⅱ is isolated from this animal for the first time,both of them have potential antineoplastic activities in vitro .