利用稻瘟霉分生孢子筛选有生物活性的海洋生物

Using conidia of Pyricularia oryzae screening for bioactive portions from marine organisms

目的 :筛选有生物活性的海洋生物并研究其活性成分。 方法 :采用稻瘟霉模型筛选海洋生物提取物的活性 ,利用多种层析手段和波谱技术对活性提取物跟踪分离并鉴定其成分。 结果 :实验发现 2 0种海洋生物醇提物和 5种水提物对稻瘟霉显示生物活性 ,从叶托马尾藻、铁钉菜和蓝斑背肛海兔中分离鉴定了 5 6个化合物 ,其中 2 8个对稻瘟霉显示活性且对不同肿瘤细胞株显示不同强度的细胞毒性 ,8个化合物对裴氏着色真菌具抑制作用。 结论

Objective:To screen for the bioactive marine organisms and to study the active constituents from them.Methods:Bioactivities of marine organisms extracts were screened by Pyricularia oryzae bioassay method and the constituents were isolated and identified by a combination of multi chromatography and spectral analysis.Results:Twenty ethanolic extracts and 5 aqueous extracts of the tested marine organisms showed activities causing morphological abnormality of P.oryzae mycelia.Fifty six compounds were obtained and identified from Sargassum carpophyllum,Ishige okamurai and Natarchus leachii freeri ,among which 28 compounds showed activities against P.oryzae and exhibited cytotoxicities on various cultured tumor cell lines,eight compounds showed inhibition effects on Fonsecaea pedrosoi in vitro .Conclusion:The screening data and bioactivity evaluation suggest the P.oryzae bioassay is suitable for preliminary screening of bioactive agents from marine organisms.