白细胞介素12对抗原诱导小鼠过敏性气道反应的调节作用

Modulation of antigen-induced airway allergic inflammation in mice by interleukin-12

目的 :探讨气道内应用白细胞介素 12 ( IL - 12 )对抗原诱导的过敏性气道反应的影响。方法 :C5 7BL / 6小鼠以卵白蛋白( OVA )免疫建立哮喘模型 ,在主动免疫及抗原激发阶段气道内应用 IL - 12 ,观察肺泡灌洗液细胞成分、肺部淋巴细胞产生细胞因子、外周血 Ig E水平变化。 结果 :( 1)致敏阶段应用 IL - 12可明显抑制嗜酸粒细胞浸润和肺淋巴细胞分泌 IL - 5 ,降低血浆总 Ig E和抗原特异性 Ig E的水平 ;( 2 )抗原激发阶段应用 IL - 12 ,可明显抑制嗜酸粒细胞的浸润 ,抑制肺淋巴细胞产生 IL - 4、IL - 5及增强其产生 IFN-γ,但对抗原特异性 Ig E水平则无明显影响 ;( 3 )当在致敏和激发阶段均应用 IL - 12时 ,可明显抑制肺淋巴细胞产生IL - 4、IL - 5 ,增强 IFN-γ的产生 ,抑制气道内嗜酸粒细胞的浸润以及血浆中 Ig E水平的升高。 结论 :气道内应用 IL - 12对抗原诱导的气道过敏性炎症有明显的调节作用 ,并与应用时机有关 ;为气道应用 IL -

Objective: To investigate the role of interleukin 12 (IL 12) administrated intratracheally at different phases on antigen induced airway reactions. Methods: Interleukin 12 were administrated at sensitization and challenge phase in an asthma model established in C57BL/6 mice by OVA immunization. The cellular composition of bronchoalveolar lavage fluid, the cytokines secreted by lung lymphoid cells and serum IgE level were analyzed. Results: (1) In allergic sensitization phase, IL 12 strikingly inhibited the IL 5 production from lung lymphoid cells, the total OVA specific IgE levels at sera, and the BAL eosinophilia following antigen challenge. (2) During antigen challenge phase, IL 12 inhibited the recruitment of eosinophils in the airway, down regulated the IL 5, and upregulated interferon γ (IFN γ), but had no effect on IgE levels. (3) When administrated in both sensitization phase and challenge phase, IL 12 down regulated IL 4 and IL 5 production from lung lymphoid cells and IgE levels in serum, inhibited the eosinophlia and increased IFN γ expression. Conclusion: IL 12 administrated intratracheally has significant immunomodulatory effects on allergic lung inflammation, providing a new approach to use IL 12 in treatment of asthma