胃癌组织中树突状细胞浸润及其表面分子表达的临床意义

Clinical significance of dendritic cell infiltration and surface molecules expression in gastric cancer

目的 :研究胃癌组织中树突状细胞 ( dendritic cells,DC)浸润及其表面分子表达的临床意义。方法 :采用酶消化胃癌组织 ,分离细胞后进行免疫组化 S- 10 0染色和流式细胞术分析 ,研究 15例胃癌组织中 DC的浸润及其表面分子表达与胃癌病理分期的关系 ,并通过 RT- PCR技术分析了 IL - 10、VEGF和 TGF-β1 等免疫抑制因子在胃癌细胞中的表达。 结果 :胃癌组织中 DC的数量少 ,而且其表面分子如 MHC 类分子、共刺激分子 B7- 1和粘附分子 ICAM- 1等呈低表达或不表达 ,DC的数量及其表面分子的表达与胃癌的病理分期负相关。免疫抑制因子 IL- 10、VEGF和 TGF- β1 在胃癌细胞中有高水平的表达。 结论 :提示胃癌组织中

Objective:To investigate the clinical significance of dendritic cell(DC) infiltration and molecules expression in gastric cancer.Methods:After tumor mass was digested by enzyme and cells were seperated,anti S 100 protein antibody immunohistochemical staining and flow cytometric analysis were done in 15 cases of gastric cancer.Reverse transcription polymerase chain reaction(RT PCR)was used to detect IL 10,VEGF and TGF β 1 mRNA expression in gastric cancer cells.Results:The number of DC in gastric cancer was just small, and correlated reversely with pathologic stages.The levels of some surface molecules expression such as MHC class Ⅱ, costimulatory molecules B7 1 and ICAM 1 in gastric cancer were negative.The number of DC and the levels of some surface molecules expression in gastric cancer were correlated reversely with pathologic stages.Levels of IL 10,VEGF and TGF β 1 mRNA expression in gastric cancer cells were high.Conclusion:The results indicate that infiltration and surface molecules expression in gastric cancer are closely linked to tumor pathologic stages.