补肾中药对老年大鼠脑组织中胆碱能M受体分布的影响

Effect of Kidney-tonifying Traditional Chinese Medicine on Distribution of Cholinergic Muscarinic Receptor in Brain Tissue of Aging Rats

采用受体放射配基结合分析、原位杂交和放射自显影术等方法 ,研究胆碱能M受体、M1受体mRNA在脑组织中的分布 ,观察补肾中药对胆碱能M受体的含量、功能及分布的影响 ,探讨补肾中药延缓脑组织衰老的机理。结果显示 :老年大鼠大脑皮层、海马、纹状体中M受体明显减少 ,放射自显影的灰度值降低 ,胆碱能M 1受体mRNA在老年大鼠纹状体部位的表达减少 ,在其他部位的不明显。补肾中药明显增加老年大鼠胆碱能M受体的数量 ,对脑内胆碱能M 1受体mRNA转录水平降低的部位有改善作用。补肾中药可通过改善胆碱能M1受体的mRNA表达、增加脑内胆碱能M 1受体数量来延缓脑组织衰老。

The distribution of cholinergic Ml-R mRNA and M-R in brain tissue was studied by means of receptor radioligand binding assay, in situ hybridization and autoradiograph, to observe the effect of kidney-tonifying TCM on the content, function and distribution of M-R, then discussing the anti-aging mechanism of kidney-tonifying TCM. The results were: (1) the content of M-R in brain cortex, hippocampus and striatum of aging rats was decreased, and the kidney-tonifying TCM can increase the amount of M-R in aging rats; (2) the express of the M1-R mRNA was significantly decreased in the striatum of aging rats, in the other region of the brain the change is not obvious. The kidney-tonifying TCM can improve the transcription of M1-R mRNA. Its suggested that the kidney-tonifying TCM can delay the brain senescence by improving the express of M1-R mRNA, increasing the amount of muscarinic receptor in aging rats. Thus our study offered an experimental basis for kidner-tonifying TCM preventing and treating aging.