草原毛虫核型多角体病毒的安全性研究 Ⅱ.用Ames法检测核型多角体病毒的致突变性

STUDIES ON THE MUTAGENICITY OF GrNPVs ON VERTEBRATE Ⅱ.THE MUTAGENICITY OF GrNPVs EXAMINED BY AMES TEST

用Ames法检测了GrNPV的致突变性.实验用常规的点试法,不经代谢活化和经代谢活化的渗入法,和改良的平板渗入法来检测不同剂量的GrNPV对原核生物的致突变性.结果表明,GrNPV对原核生物无诱变作用,无剂量效应关系,从而推测GrNPV对哺乳动物无潜在的致突变性.

The mutagenicity of GrNFV was studied by using Ames Test, The point test, osmosis test and liquid preincubation tost were adopted in this experiment. The results show that the GrNPVs examined have no mutagenesis effect on procaryote, so we can infer that GrNPVs have no potential mutagenesis effect on vertebrate.