血链菌死菌在生物膜再形成中的作用观察

The Role of Dead Bacteria in Streptococcus Sanguis Biofilm Reformation

目的 探讨血链菌死菌在生物膜更新形成中的作用。方法 利用激光共聚焦扫描显微镜和死菌 /活菌荧光染色技术相结合 ,对人工口腔内覆盖死菌 (实验组 )和未覆盖死菌 (对照组 )盖玻片表面血链菌生物膜的形成进行动态观察。结果 血链菌生物膜形成 3 0min时 ,实验组生物膜厚度和对照组相比 ,无显著性差异 ;但其细菌密度明显大于对照组 (P <0 .0 5 ) ,至 60min ,12 0min ,2 4 0min时 ,实验组生物膜厚度和对照组相比 ,有显著性差异 (P <0 .0 5 ) ,但其细菌密度几乎一致。结论 在生物膜的形成初期 ,死菌具有一定的促进作用。

Objective To investigate the role of dead bacteria in Streptococcus sanguis biofilm reformation.Methods Vital fluorescent staining technique and Confocal laser Scanning Microscopy(CLSM) were combined to observe Streptococcus sanguis biofilm on the saliva coated glass(SCG) in an artificial mouth model, the thickness and bacterial density were compared between biofilm reformed on saliva covered slide with dead cells (test group)and biofilm reformed on saliva coated slide without dead cells(control group) at different times.Results At 30min, the bacterial density rather than biofilm thickness of test group was significantly higher than that of control group ( P <0.05),whereas at 60min,120min,240min,the biofilms were significantly thicker in test group than that in control group ( P <0.05).Conclusion The dead cells may help biofilm reformation during the early formation of biofilm.