多孔块状β-磷酸三钙兔颅骨骨膜下埋植引导成骨的组织学观察

A Histologic Observation of Bone Formation Induced by Subperiosteal Implantation of Resorbable Porous Block β-Tricalcium Phosphate on Calvarial Surface in Rabbits

目的 通过组织切片观察 ,了解多孔块状 β-磷酸三钙 ( β TCP)在皮质骨表面引导成骨过程中的组织学表现及可吸收机理。方法 将多孔块状 β TCP陶瓷在兔颅骨骨膜下埋植 ,实验标本常规石蜡切片 ,HE染色和Masson三色染色 ,显微镜下观察各组标本的组织学表现 ,并和羟基磷灰石 (HA)作比较。结果 β TCP及HA陶瓷均能引导新骨生成 ,新骨大量从颅骨表面长入材料内 ,颅骨骨膜下无明显成骨 ,并观察到大量多核异物巨细胞吞噬 β TCP的现象。结论 β TCP能引导大量新骨生成 ,可以作为萎缩牙槽嵴加高的代用品。细胞对材料的吞噬作用是 β

Objective By the histologic observation of samples, understanding the osteoconductive histologic behaviour and resorbable mechanism ofβ tricalcium phosphate(β TCP).Methods Porous blockβ TCP samples were implanted into calvarial surface.Tissue slices dyed with methods of HE and Masson's were observed by microscope and compared with hydroxyapatite(HA).Results β TCP had the osteoconductivity, as well as HA, plentiful new bone penetrated into materials from the calvarial surface.There was no new bone under the periost obviously. A large number of rhagiorine cells swallowing β TCP had been observed.Conclusion β TCP has osteoconductivity ,can be used as a substitution for atrophic alveolar ridge augmentation. The function of rhagiorine cell swallowingβ TCP is the essential resorbable mechanism of β TCP.