苯异丙腺苷对脉冲噪声所致听觉损伤的保护作用

Effects of Impulse Noise on Internal Ear and Protection from Noise-induced Internal Ear Damage by R-N6-phenylisopropyladenosine(R-PIA)

[目的 ]研究听觉系统在脉冲噪声作用下的损伤特点以及在脉冲噪声环境下苯异丙腺苷 (R phenylisopropy ladenosine ,R PIA ,见SigmaChemicals)对听觉系统的保护作用。 [方法 ]将 6只灰鼠 (chinchillas)暴露于 10 0个 15 0dBSPL脉冲噪声中 ,暴露前 2h在其右耳用药 (R PIA 0 1mmol/L ,3 0 μl) ,左耳为对照。脉冲噪声是模拟M 16来福枪而成。[结果 ]对照组 (左耳 )的永久性阈移 (PTS)在 2 2～ 40dB之间 ,用药组 (右耳 )的PTS比对照组少 8～ 13dB。其用药组的外、内毛细胞缺失率明显比对照组少 (10～ 5 0 %、15～ 45 % )。 [结论 ] 15 0dBSPL的脉冲噪声所造成的听觉系统损伤大大超过等能量的稳态噪声 ;R

To study the damage of auditory system by impulse noise and the protection of auditory system from impulse noise damage by prior treatment with R phenylisopropyladenosine. This experiment had 6 chinchillas right ears pre treated with R PIA(0 1 mmol/L 30 μl) 2 hours before exposure to 100 impulses at 150 dB peak SPL. Left ears were as controls. These impulses mimicked the waveform of an M 16 rifle fire. R PIA attenuated noise induced permanent threshold shifts(PTS) by 8～13 dB,as well as missing rate of outer and inner hair cells by 10～50% and 15～45%,respectively. [Conclusion] The damage by impulse noise at 150 dB peak SPL was much severer than that by steady state noise with equivalent energy;R PIA may be effective in protection of auditory system from impulse noise damage.