维生素E对染镍肺泡巨噬细胞膜脂质过氧化的影响

Effects of Vitamin E on the Lipid Peroxidation and Antioxidant Enzyme in Alveolar Macrophages Treated with Ni_2O_3

[目的 ]探讨维生素E拮抗镍所致细胞毒作用及作用机制。 [方法 ]采用体外细胞培养方法 ,观察维生素E对染镍肺泡巨噬细胞膜脂质过氧化产物丙二醛 (MDA)及超氧化物歧化酶 (SOD)活性的影响。 [结果 ]在体外染镍肺泡巨噬细胞培养过程中加入不同浓度的维生素E(2 5、5 0或 10 0 μmol/L)可提高肺泡巨噬细胞内SOD活性 ,具有剂量反应关系。维生素E能显著抑制MDA生成量 ,其中以 5 0和 10 0 μmol/L浓度组作用最为明显。[结论 ]镍可增加细胞脂质过氧化 ,维生素E具有拮抗镍所致细胞毒性的作用 。

To investigate the effects of vitamin E on the injury of alveolar macrophages treated with Ni 2O 3. The alveolar macrophages were cultured in vitro with exposure to Ni 2O 3 .Meanwhile,vitamin E at a concentration of 25,50 or 100μmol/L was added into the medium,respectively.The detection of malondialdehyde and activity of antioxidant enzyme in alveolar macrophages were carried out. The results showed that vitamin E could increase the activity of superoxide dismutase in alveolar macrophages in a dose dependent manner.The vitamin E at 50μmol/L and 100μmol/L had dramatic inhibitory effect on the production of malondialdehyde by alveolar macrophages.[Conclusion] Vitamin E could inhibit the cytotoxity of Ni 2O 3 probably due to its antioxidant property