摘要
本文首次制备了胰岛素氮氧自由基,研究了胰岛素探针与其受体结合过程中ESR波谱参数的动力学变化.实验表明胰岛素与其受体结合前后,膜蛋白质的构象、胰岛素分子的旋转相关时间(τ_c)和胰岛素氮氧自由基的ESR波谱振幅(h_0)均发生明显的变化,这些变化均与胰岛素-受体的内噬现象有关系.当封闭受体上的巯基、氨基或羟基时,胰岛素与其受体的结合能力便受到一定的阻抑作用.
The insulin nitroxide free radical was first prepared and used to study the kinetic changes of the spectral parameters in the process of combination between the insulit: probe and its receptor. The experiments show that before and after combination the conformation of membrane protein, rotational correlation time ( τC) of insulin molecule and the amplitude (h0) of ESR spectra of insulin nitroxide free radical have been obviously changed. These changes of parameter of ESR spectrum are related to the endocytose of insulin receptor complexes. The combination could rather be inhibited, when the sulfhydryl group, free amino group or hydroxide group of receptor were blocked.
出处
《波谱学杂志》
CAS
CSCD
1989年第3期341-346,共6页
Chinese Journal of Magnetic Resonance
关键词
胰岛素
ESR波谱
波谱
ESR spectra
Insulin
Spin labeling.
