应用PCR对病毒性心肌炎小鼠进行病原学诊断及其相关因素的研究

Study on Etiologic Diagnosis and Its Related Factors in Mice with Viral Myocarditis by PCR

目的 :研究用聚合酶链反应 (PCR)技术测定病毒性心肌炎小鼠CoxsackieVirusB3RNA(CVB -RNA)对病毒性心肌炎病毒病原的敏感性和可靠性。方法 :对 75只小鼠腹腔注射CVB3病毒后 ,做心肌病理检查和检验血液中CVB -RNA。结果 :75只小鼠病理检查结果有心肌炎改变占 85 3 % ,CVB -RNA阳性率为 5 3 3 % ;CVB -RNA阳性率 2周以内显著高于 2周以后。结论 :用PCR技术检测病毒性心肌炎血中CVB -RNA的敏感性与可靠性均较高 。

Objective:To realize the sensitivity and reliability of CVB-RNA assay in blood by PCR technique to identify viral pathogen of myocarditis.Method:CVB 3 was injected into the abdominal cavity of 75 mice.Then pathological exam and CVB-RNA assay by PCR technique was done.Result:64 of 75 mice(85.3%) had pathological change as myocarditis.CVB-RNA was positive in 40 of 75 mice(53.3%).The positive rate of CVB-RNA in myocarditis were compared in cases whose course within two week and more than two week.Conclusion:The sensitivity and reliability of CVB-RNA assay in blood by PCR technigue to identify viral pathogen of myocarditis.The method of exam is suitable for early stage of VM.