血液及血制品感染因子检测

Detection of Infective Agent from Blood and Plasma Derivatives

目的了解 1997～ 1999年度我市临床使用的血液及血液制品中甲肝病毒 (HAV)、乙肝病毒 (HBV )、丙肝病毒 (HCV)、艾滋病病毒 (HIV)及梅毒螺旋体的感染情况。方法 用 EL ISA、PCR和 TRU ST对 171份血液、83份血制品检测了抗 - HAV Ig M、HBs Ag、抗 - HCV、HCV RNA、抗 - HIV1 + 2 、TRUST、TPPA。结果 171份血液标本抗 - HAV Ig M、抗 - HCV、抗 - HIV1 + 2 皆阴性 ;HBs Ag阳性 1例 (0 .0 5 8% ) ,梅毒 1例 (0 .0 5 8% ) ;83份血制品抗 -HAV Ig M、HBs Ag、抗 - HIV1 + 2 、TRU ST均阴性 ,抗 - HCV阳性率为 2 4 .1% ,均为人血丙种球蛋白 ,取 4个批号阳性的各 2份进行 HCV- RNA检测 ,结果 6份 HCV RNA阳性。结论 1997～ 1999年度临床上所使用的血液及血制品 (人血丙种球蛋白 )仍存在残余危险 ,需采取措施减少残余危险度。

OBJECTIVE To evaluate the risk of HAV, HBV, HCV, HIV and syphilis infection from blood and plasma derivatives used in clinics during 1997 1999. METHODS Blood 171 and plasma 83 derivatives were detected for anti HAV IgM, HBsAg, HCV RNA, anit HIV 1+2 ,TRUST, TPPA by ELISA, PCR, TRUST and TPPA. RESULTS One hundred and seventy one samples of blood detected were anti HAV IgM, anti HCV, anti HIV 1+2 negative except one was HBsAg positive and other one was syphilis positive; but all samples of plasma products detected were anti HAV IgM, HBsAg, anti HIV 1+2 , TRUST negative, the positive rate of anti HCV was 24.1%. From 8 samples with anti HCV positive human immunoglobulin, the positive of HCV RNA was 6. CONCLUSIONS Results suggested that the blood and plasma products used in clinics during 1997 1999, be not always safe, we recommend further improvement to current blood screening practices.