肝纤灵对肝纤维化大鼠血清细胞因子水平的影响

EFFECT OF GANXIANLING ON SERUM CELL FACTOR OF RAT WITH LIVER FIBROSIS

目的 :研究肝纤灵的抗肝纤维化作用与血清中肿瘤坏死因子 (TNF)、白细胞介素 - 1(IL - 1)、白细胞介素 - 6 (IL - 6 )的关系。方法 :CCl4皮下注射造成大鼠肝纤维化 ,治疗组同时口服肝纤灵水煎液 ,分别于灌胃后第 3、6、9、12w ,分批分组自眼眶静脉丛取血 ,分离所得血清 ,用相应的放免试剂盒检测TNF、IL - 1,IL - 6。结果 :从第 3w开始 ,模型组大鼠血清中TNF水平出现升高 ,并在第 6w出现峰值 ,6～ 9w时与空白对照组相比有显著差异 (P <0 0 1)。肝纤灵高、低剂量组与空白对照组TNF水平相近 ,6、9w值均显著低于模型组。模型组大鼠血清中IL - 6水平明显低于正常对照组 ,IL - 1水平明显高于正常对照组 ,不同剂量治疗组则能显著改善上述趋势。结论 :肝纤灵抗肝纤维化作用与TNF、IL - 1和IL -

Objective:Investigating the relationship between the effect of ganxianling on liver fibrosis and TNF,IL-1,IL-6 in serum.Methods:Replicating the liver fibrosis model by sc.CCl 4 at the same time,rats were treated with ganxianling then,at the 3,6,9,12 weeks separatly,took serum from rat's eye socket vein clump,and tested the level of TNF,IL-1,IL-6 in serum using corresponding kits.Results:At teh beginning of 3 weeks,the level of serum TNF of model rats improved gradually,and appeared peak value at 6 weeks.this level was evidently higher than that of normal group.On the other hand,treated with ganxianling could significantly ameliorate this change.It also could be found that the level of IL-6 and IL-1 in serum of model rats evidently lowered or hoisted separatly than that of normal,and treated with different dosage of ganxianling could evidently ameliorate the above trends.Conclusion:The effect of ganxianling on liver fibrosis was direct correlation with cell factors such as TNF,IL-1 and IL-6.