阿克拉霉素影响骨髓增生异常综合征细胞株细胞增殖及其机制的初步研究

Effect of aclacinomycin on proliferation of myelodysplastic cell line MUTZ-1 and its mechanism

目的 探讨阿克拉霉素 ( aclacinomycin,ACM)对骨髓增生异常综合征 ( myelodysplastic syndrome,MDS)细胞株 ( MUTZ- 1)细胞的体外作用及其机制 ,以期为进展期 MDS的治疗提供理论基础。方法 采用 MTT比色法、流式细胞术、DNA凝胶电泳法研究 ACM对 MU TZ- 1细胞生长的影响。结果 ( 1) ACM对 MUTZ- 1细胞的生长具有抑制作用 ,其 4 8小时 IC50 为 0 .4 6μmol。( 2 ) ACM对 MUTZ- 1细胞的作用主要为诱导凋亡。( 3 ) ACM诱导 MUTZ- 1细胞凋亡 ,阻滞细胞于细胞周期的 G2 / M期。结论 ACM可诱导 MDS细胞株细胞凋亡 ,作用靶点位于 G2 / M期。提示诱导细胞凋亡疗法治疗进展期

Objective To explore the effect of aclacinomycin (ACM) on the proliferation of myelodysplastic syndrome (MDS) cell line (MUTZ 1) and its mechanism. Methods MUTZ 1 cell growth was measured by MTT assay, cell apoptosis was investigated by flow cytometry and DNA gel electrophoresis. Results (1)ACM suppressed the growth of MUTZ 1 cells and the IC 50 was 0.46 μmol at 48 h. (2)Inducing apoptosis was the main effect of ACM on MUTZ 1 cells. (3)ACM arrested MUTZ 1 at G2/M phase of cell cycle. Conclusion ACM can induce MDS cell apoptosis and the cell cycle was arrestcd at G2/M phasc which suggests the feasibility of inducing apoptosis in advanced MDS treated by ACM.\;