凋亡抑制基因bcl-2产物在睾丸肿瘤中的表达及其意义

Immunohistochemical Study of Apoptosis-Suppressing Oncoprotein bcl-2 in Human Testicular Tumors

目的 :探讨凋亡抑制基因bcl 2产物表达与睾丸肿瘤生物学行为及预后的关系。方法 :采用免疫组化S P法对 32例睾丸肿瘤和 10例正常睾丸组织中的bcl 2表达产物进行定量检测。结果 :81.3% (2 6 32 )睾丸肿瘤显示阳性反应 ,阳性细胞率为 13.96 % ,正常睾丸组织为阴性反应 ,bcl 2蛋白在正常睾丸组织和肿瘤组织中的表达有显著差异性 (P <0 .0 5 )。bcl 2表达与睾丸肿瘤病理分级、临床分期有关 ,低分化组阳性细胞率显著低于高分化组 (P<0 .0 5 )。bcl 2表达随着睾丸肿瘤临床分期的上升阳性细胞率降低 ,Ⅰ期与Ⅲ期间bcl 2阳性细胞率有显著差异性(P <0 .0 5 )。bcl 2表达在精原细胞瘤及非精原细胞瘤间无显著性差异 (P>0 .0 5 )。结论 :提示bcl 2基因可能与睾丸肿瘤的发生、发展有关 。

Objective:To find the correlation between bcl 2 and the biological behaviors and prognosis of testicular tumor. Methods: 32 human testicular tumors and 10 normal testicular tissue specimens had been studied by S P immunohistochemical detection.Results:There was no positive expression in normal testicular tissues or in normal testicular tissues adjacent to neoplastic lesions while bcl 2 protein expression was demonstrated in 81.3% of tumors. bcl 2 protein expression was relevant to the tomor grade and clinical stage.The positive expression in high grade tumors was significantly lower than that in low grade tumors(P<0.05).And the positive expression in low stage(Ⅰ) was also significantly higher than that in the high stage tumors(Ⅲ) (P<0.05). There was no significant difference between seminoma and nonseminoma(P>0.05). Conclusion: bcl 2 might play a critical role in the genesis and development of tesiticular tumor and could be applicable as a tumor marker for the evaluation of malignant potential and prognosis of testicular tumor.