二苯胺法定量检测细胞凋亡

The Apoptosis Quantitative Detection by Diphenylamine

目的 :建立二苯胺定量检测细胞凋亡的实验方法。方法 :以地塞米松体外作用 5h的小鼠胸腺细胞作为凋亡阳性细胞 ,应用二苯胺法测定细胞片段化DNA和总DNA的含量 ,二者的比值定义为细胞凋亡率。结果 :地塞米松 (1× 10 - 5mol L)处理 5h后 ,细胞凋亡率为 30 .2 4 %。此与流式细胞仪分析结果 (2 7.10 % )基本一致。结论 :建立了一种简单、经济。

Objective: To establish the method of the apoptosis quantitative detection by diphenylamine. Methods:The apoptosis of separated mice thymocytes were induced by treating 5 hours of dexamethasone (1×10 -5 mol/L). The ratio of DNA fragments to the total of DNA detected by diphenylamine method was defined to the positive rate of apoptosis. Results:The apoptosis positive rate of mice thymocytes was 30.24% after treated 5 hours by dexamethasone ,which was close to the result of flow cytometry analysis(27.10%). Conclusion:We have built a simple, cheap and rapid method for quantitative detection of apoptosis.