非霍奇金淋巴瘤与p53蛋白表达的关系

Relationship between non-Hodgkin's lymphoma and p53 protein expression

目的 :探讨非霍奇金淋巴瘤 (NHL)与 p5 3蛋白表达的关系。 方法 :用免疫组化S P法检测 10 2例 (低度恶性 2 3例 ,中度恶性 36例 ,高度恶性 4 3例 )NHLp5 3蛋白表达 ,根据 p5 3蛋白阳性细胞百分率将其表达水平分为 4级 :0级 (阴性 ) ,1级 (1%～ 2 5 % ) ,2级 (2 6 %～ 5 0 % ) ,3级 (>5 0 % )。结果 :低度恶性组 2 0 / 2 3(87% )p5 3表达为 0级 ,中度恶性组 31/ 36 (86 1% )表达为 1级 ,高度恶性组 33/ 4 3(76 7% )表达为 2～ 3级。 2 5例随访 7～ 6 8个月 ,p5 30～ 1级NHL完全缓解率 (CRR ,11/ 14 )高于p5 32～ 3级NHLCRR (1/ 11,P <0 0 1) ,前者生存率 (13/ 14 )高于后者 (3/ 11,P <0 0 1)。NHLp5 3蛋白表达水平与其恶性度密切相关 (P <0 0 1)。结论 :p5 3蛋白表达阳性细胞百分率是判断NHL恶性度、疗效及预后较可靠的参数。肿瘤性p5

Purpose To study the relationship between non Hodgkin's lymphoma(NHL) and p53 expression. Methods One hundred and two cases of NHL (low grade 23 cases, intermediate grade 36 cases, high grade 43 cases) were examined with immunohistochemical staining for p53 oncoprotein. Expression levels were divided into 4 degrees with the percentage of positive cells for p53: degree 0(negative), degree Ⅰ (1%～25%), degree Ⅱ (26%～50%), degree Ⅲ (>50%). Results Expression levels of p53 were strongly associated with the grade of malignancy ( P< 0 01). 25 cases were followed up for 7～68 months, the complete remission rate (11/14) in degrees 0 and Ⅰ NHL was higher than that (1/11) in degrees Ⅱ and Ⅲ NHL ( P< 0 01). The survival rate (13/14) in the former was higher than that (3/11) in the latter ( P< 0 01). Conclusions The percentage of positive cells for p53 in NHL is a reliable parameter for predicting the grade of malignancy, the curative effect and the prognosis of patients with NHL. It plays an important role in the diagnosis of intermediate high grade NHL to detect p53 oncoprotein in tissue sections.